Shape and compliance of endothelial cells after shear stress in vitro or from different aortic regions: scanning ion conductance microscopy study.

PloS One
Claire M F PotterJulia Gorelik

Abstract

To measure the elongation and compliance of endothelial cells subjected to different patterns of shear stress in vitro, and to compare these parameters with the elongation and compliance of endothelial cells from different regions of the intact aorta. Porcine aortic endothelial cells were cultured for 6 days under static conditions or on an orbital shaker. The shaker generated a wave of medium, inducing pulsatile shear stress with a preferred orientation at the edge of the well or steadier shear stress with changing orientation at its centre. The topography and compliance of these cells and cells from the inner and outer curvature of ex vivo porcine aortic arches were measured by scanning ion conductance microscopy (SICM). Cells cultured under oriented shear stress were more elongated and less compliant than cells grown under static conditions or under shear stress with no preferred orientation. Cells from the outer curvature of the aorta were more elongated and less compliant than cells from the inner curvature. The elongation and compliance of cultured endothelial cells vary according to the pattern of applied shear stress, and are inversely correlated. A similar inverse correlation occurs in the aortic arch, with variation b...Continue Reading

References

May 1, 1991·Journal of Biomechanical Engineering·G HelmlingerR M Nerem
Feb 3, 1989·Science·P K HansmaC B Prater
May 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M SatoR M Nerem
Nov 1, 1985·Journal of Biomechanical Engineering·M J Levesque, R M Nerem
Jul 1, 1984·Microvascular Research·S G EskinL T Navarro
Aug 1, 1981·Journal of Biomechanical Engineering·C F DeweyP F Davies
Nov 22, 1997·Journal of Microscopy·Y E KorchevM J Lab
May 26, 2001·The Journal of Clinical Investigation·M I CybulskyD S Milstone
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Konstantin G BirukovJoe G N Garcia
Jun 8, 2002·Annals of Biomedical Engineering·Brian P Helmke, Peter F Davies
Nov 24, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Jin SuoDon P Giddens
Sep 15, 2007·American Journal of Physiology. Cell Physiology·Rosalind E Mott, Brian P Helmke
Jun 17, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Ryan E FeaverBrett R Blackman
Feb 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·H OberleithnerH E de Wardener
Mar 3, 2009·Nature Methods·Pavel NovakYuri E Korchev
Dec 4, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Claire M F PotterJane A Mitchell
Jan 6, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Andrew R BondPeter D Weinberg
Mar 12, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Alexis BroisatLaurent M Riou

❮ Previous
Next ❯

Citations

Dec 4, 2012·Sensors·Patrick HappelIrmgard D Dietzel
Aug 1, 2013·Journal of Materials Science. Materials in Medicine·Alan BrownBrian J Meenan
Aug 21, 2014·Annals of Biomedical Engineering·Yumnah MohamiedPeter D Weinberg
Oct 23, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Max J LabJulia Gorelik
Nov 8, 2014·Journal of Biomechanical Engineering·Caleb A DavisMichael Moreno
Sep 10, 2017·Australasian Physical & Engineering Sciences in Medicine·Anthony MazzaJames Ziegler
Jul 30, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Mean GhimPeter D Weinberg
May 6, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Michele MiragoliAdrian H Chester
Apr 2, 2021·Circulation Research·Pierre BoutouyrieGary F Mitchell
Aug 20, 2021·Advanced Healthcare Materials·Yuji NashimotoHitoshi Shiku
Dec 10, 2020·Chemical Reviews·Cheng ZhuLane A Baker
Oct 7, 2021·Biotechnology and Bioengineering·Mehwish ArshadPeter D Weinberg

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
Scanning Ion Conductance Microscopy
AFM

Software Mentioned

ScanIC Image
SICM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.