Shape-Dependent Biomimetic Inhibition of Enzyme by Nanoparticles and Their Antibacterial Activity

ACS Nano
Sang-Ho ChaNicholas A Kotov

Abstract

Enzyme inhibitors are ubiquitous in all living systems, and their biological inhibitory activity is strongly dependent on their molecular shape. Here, we show that small zinc oxide nanoparticles (ZnO NPs)-pyramids, plates, and spheres-possess the ability to inhibit activity of a typical enzyme β-galactosidase (GAL) in a biomimetic fashion. Enzyme inhibition by ZnO NPs is reversible and follows classical Michaelis-Menten kinetics with parameters strongly dependent on their geometry. Diverse spectroscopic, biochemical, and computational experimental data indicate that association of GAL with specific ZnO NP geometries interferes with conformational reorganization of the enzyme necessary for its catalytic activity. The strongest inhibition was observed for ZnO nanopyramids and compares favorably to that of the best natural GAL inhibitors while being resistant to proteases. Besides the fundamental significance of this biomimetic function of anisotropic NPs, their capacity to serve as degradation-resistant enzyme inhibitors is technologically attractive and is substantiated by strong shape-specific antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), endemic for most hospitals in the world.

References

Nov 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C De RoeP Baudhuin
Jun 30, 1994·Nature·R H JacobsonB W Matthews
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nicholas O FischerVincent M Rotello
Oct 30, 2003·Journal of the American Chemical Society·Nicholas O FischerVincent M Rotello
Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Alexey A VertegelJonathan S Dordick
Oct 19, 2004·Cancer Letters·Thambi Dorai, Bharat B Aggarwal
Oct 28, 2004·Journal of the American Chemical Society·Ayush VermaVincent M Rotello
Jun 14, 2005·Comptes rendus biologies·Brian W Matthews
Sep 15, 2005·Journal of the American Chemical Society·Chang-Cheng YouVincent M Rotello
Jan 31, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Pratibha PandeyBansi D Malhotra
Nov 24, 2007·Science·David R Dodds, Richard A Gross
Feb 16, 2008·Journal of the American Chemical Society·Nikolaos G ChalkiasEmmanuel P Giannelis
Oct 22, 2008·Accounts of Chemical Research·Tsuyoshi Takahashi, Hisakazu Mihara
Oct 31, 2008·Chemistry & Biodiversity·Shaofen GuoXiaohua Huang
Jan 10, 2009·Journal of the American Chemical Society·Marko GolicnikFlorian Hollfelder
Dec 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Soumyananda ChakrabortiPinak Chakrabarti
Jan 22, 2010·Journal of the American Chemical Society·Ming YangNicholas A Kotov
Oct 12, 2010·Science·Nicholas A Kotov
Mar 16, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Krishna R RaghupathiAdhar C Manna
Jun 2, 2011·Journal of the American Chemical Society·Oscar R MirandaVincent M Rotello
Nov 18, 2014·Nature Materials·Jihyeon YeomNicholas A Kotov
Jan 30, 2015·Nature·Joong Hwan BahngNicholas Kotov

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Citations

May 1, 2016·Trends in Plant Science·Peng WangPeter M Kopittke
Jun 10, 2016·Nanoscale·Sivakoti SangabathuniRaghavendra Kikkeri
Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Khan Behlol Ayaz AhmedAnbazhagan Veerappan
Nov 9, 2017·Nanomaterials·Jayden McCallRobert K DeLong
Jun 12, 2018·Chemistry : a European Journal·Koushik Sarkar, Parthasarathi Dastidar
Oct 16, 2019·World Journal of Microbiology & Biotechnology·Lilit Gabrielyan, Armen Trchounian
Apr 24, 2020·Chemical Communications : Chem Comm·Manuel González-CuestaJosé M García Fernández
Sep 16, 2020·Nanoscale·Rodrigo S FerreiraAlioscka A Sousa
Jul 28, 2016·Shock·J Scott VanEpps, John G Younger
Apr 19, 2017·FEMS Microbiology Reviews·Michal Natan, Ehud Banin
Apr 2, 2019·Nanoscale Advances·André L LiraAlioscka A Sousa
Jul 18, 2018·Frontiers in Microbiology·Pedro V BaptistaAlexandra R Fernandes
Aug 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Thi Minh Nguyet TrinhJean-François Nierengarten
Aug 7, 2019·Current Drug Metabolism·Ayse Basak Engin, Atilla Engin
Oct 31, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Raghvendra Pratap SinghGeetanjali Manchanda
Feb 20, 2021·International Journal of Nanomedicine·Yaling HuangJuqun Xi
Mar 26, 2021·Heliyon·Shabnam SharminOluyomi Stephen Adeyemi
Nov 9, 2018·International Journal of Biological Macromolecules·Mansoore Hosseini-KoupaeiZahra Enteshari
Sep 2, 2017·Chemical Reviews·Calum KinnearAlke Petri-Fink
Mar 16, 2017·ACS Nano·Beatriz PelazWolfgang J Parak

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