Shared and distinct transcriptomic cell types across neocortical areas

Nature
Bosiljka TasicHongkui Zeng

Abstract

The neocortex contains a multitude of cell types that are segregated into layers and functionally distinct areas. To investigate the diversity of cell types across the mouse neocortex, here we analysed 23,822 cells from two areas at distant poles of the mouse neocortex: the primary visual cortex and the anterior lateral motor cortex. We define 133 transcriptomic cell types by deep, single-cell RNA sequencing. Nearly all types of GABA (γ-aminobutyric acid)-containing neurons are shared across both areas, whereas most types of glutamatergic neurons were found in one of the two areas. By combining single-cell RNA sequencing and retrograde labelling, we match transcriptomic types of glutamatergic neurons to their long-range projection specificity. Our study establishes a combined transcriptomic and projectional taxonomy of cortical cell types from functionally distinct areas of the adult mouse cortex.

References

Jan 25, 2019·Journal of Anatomy·Robert F Hevner
Mar 13, 2019·The Journal of Comparative Neurology·Michael N EconomoJayaram Chandrashekar
Jun 22, 2019·The Journal of Comparative Neurology·Jana HedrichHeiko J Luhmann
Jul 25, 2019·Evolution; International Journal of Organic Evolution·Lucie MarhounováPavel Němec
Nov 1, 2019·Nature·Julie A HarrisHongkui Zeng
Nov 13, 2019·FEBS Letters·Larissa TraxlerJerome Mertens
Nov 4, 2019·The Journal of Cell Biology·Christina GöngrichCarlos F Ibáñez
Dec 21, 2019·ELife·B Semihcan SermetCarl C H Petersen
Jun 8, 2019·Journal of Anatomy·Noemi PiccoThomas E Woolley
Feb 7, 2020·PLoS Biology·Amir DudaiMichael London
Feb 12, 2019·Nature Methods·George C LindermanYuval Kluger
Feb 12, 2020·ELife·Michael L WallaceBernardo L Sabatini
Feb 20, 2020·The Journal of Comparative Neurology·Attilio IemoloFrancesca Telese
Feb 25, 2020·PLoS Computational Biology·Kael DaiAnton Arkhipov
Jan 24, 2020·Bioinformatics·Shobana V StassenKevin K Tsia
May 2, 2020·The American Journal of Psychiatry·Timothy Spellman, Conor Liston
Nov 7, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xia LeiG William Wong
Jun 22, 2019·Nature Reviews. Neuroscience·Z Josh Huang, Anirban Paul
Jul 13, 2019·Annual Review of Neuroscience·Gord Fishell, Adam Kepecs
Jul 16, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Veronica B Searles QuickMatthew W State
Jun 23, 2020·Human Molecular Genetics·Stefano BertoGenevieve Konopka
Sep 5, 2019·Nature·Matthew G Keefe, Tomasz J Nowakowski
Aug 21, 2020·Physiological Reviews·Jochen Staiger, Carl C H Petersen
May 26, 2020·Neuroinformatics·Nestor TimonidisPaul Tiesinga
Jul 15, 2020·Bioinformatics·Antti HäkkinenSampsa Hautaniemi
Apr 6, 2019·Frontiers in Neuroanatomy·Gordon M ShepherdGiorgio A Ascoli

Citations

Jul 8, 1988·Science·P Rakic
Mar 20, 2003·Brain Research. Developmental Brain Research·Robert F HevnerAndy Fink
Dec 22, 2005·Nature Neuroscience·Ken SuginoSacha B Nelson
Jan 18, 2006·Development·Michio YoshidaElizabeth A Grove
May 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thomas S HnaskoRichard D Palmiter
Jun 30, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cecilia BondjersChrister Betsholtz
Dec 8, 2006·Nature·Ed S LeinAllan R Jones
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Santo Fortunato, Marc Barthélemy
Jan 18, 2007·PloS One·Christopher S Raymond, Philippe Soriano
Feb 3, 2007·BioTechniques·Michael R LamprechtAnne E Carpenter
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sophia H L GeorgeAndras Nagy
May 22, 2007·Nature Reviews. Neuroscience·Bradley J MolyneauxJeffrey D Macklis
Oct 30, 2007·Neuron·Dennis D M O'LearySetsuko Sahara
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John D CahoyBen A Barres
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Feb 17, 2009·Nature Neuroscience·Lydia NgMichael Hawrylycz
Mar 19, 2009·Cerebral Cortex·Albert E Ayoub, Ivica Kostovic
Mar 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jessica A CardinLarry A Palmer
Dec 15, 2010·Developmental Neurobiology·Bernardo RudyJens Hjerling-Leffler
Aug 16, 2011·Developmental Cell·Annika ArmulikChrister Betsholtz
Jul 24, 2012·Nature Biotechnology·Daniel RamsköldRickard Sandberg
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Nov 28, 2012·Science·Hiroki TaniguchiZ Josh Huang
Jan 5, 2013·Science·Le CongFeng Zhang
May 10, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tou Yia VueYasushi Nakagawa
Aug 21, 2013·PLoS Computational Biology·Michael LawrenceVincent J Carey
Sep 24, 2013·Nature Methods·Simone PicelliRickard Sandberg
Dec 24, 2013·Neuron·Zengcai V GuoKarel Svoboda
Apr 4, 2014·Nature·Seung Wook OhHongkui Zeng
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·María PedrazaJuan A De Carlos
Jul 30, 2014·Frontiers in Neural Circuits·Julie A HarrisHongkui Zeng
Sep 5, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ye ZhangJian Qian Wu
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Jan 28, 2015·Nature Neuroscience·Kenneth D Harris, Gordon M G Shepherd
Mar 4, 2015·Nature·Nuo LiKarel Svoboda
Oct 10, 2015·Cell·Henry MarkramFelix Schürmann

Related Concepts

Single-Cell Analysis
Study
Cortex Bone Disorders
Adrenal Cortex Diseases
Sequence Determinations, RNA
Neurons
Tissue Specificity
Structure of Cortex of Kidney
Neocortex
Lateral

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Researcher Network:CZI Neurodegeneration Challenge

The Neurodegeneration Challenge Network aims to provide funding for and to bring together researchers studying neurodegenerative diseases. Find the latest research from the NDCN grantees here.

Allen Institute for Brain Science Network

The Allen Institute for Brain Science Network is a not-for-profit biomedical research organization that provides open access to multiple neuroscience tools and resources. Find the latest research from tjr Allen Institute for Brain Science Network here.