SHB and angiogenic factors promote ES cell differentiation to insulin-producing cells

Biochemical and Biophysical Research Communications
Johan SaldeenMichael Welsh

Abstract

The potential use of embryonic stem (ES) cells for cell therapy of diabetes requires improved methods for differentiation and isolation of insulin-producing beta-cells. The signal transduction protein SHB may be involved in both angiogenesis and beta-cell development. Here we show that cells expressing the pancreatic endodermal marker PDX-1 appear in the vicinity of vascular structures in ES cell-derived embryoid bodies (EBs) cultured in vitro. Moreover, overexpression of SHB as well as culture of EBs in presence of the angiogenic growth factors PDGF or VEGF enhanced the expression of PDX-1 and/or insulin mRNA. Finally, expression of GFP under control of the PDX-1 promoter in EBs allowed for the enrichment by FACS of cells expressing PDX-1, C-peptide, and insulin as determined by immunofluorescence. It is concluded that SHB and angiogenic factors promote the development of cells expressing PDX-1 and insulin in EBs and that such cells can be separated by FACS.

References

Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L DeltourD Bucchini
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·C Garlanda, E Dejana
Dec 4, 1998·Diabetes·H Edlund
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SchuldinerN Benvenisty
Jul 27, 2001·Diabetes·S AssadyM Tzukerman
Nov 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuichi HoriSeung K Kim
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Przemyslaw BlyszczukAnna M Wobus
Nov 18, 2003·Immunological Reviews·A M James ShapiroJonathan R T Lakey
Dec 10, 2003·Experimental Hematology·Margaret H Baron
May 6, 2004·Seminars in Cell & Developmental Biology·Markus StoffelRoger A Pedersen
May 22, 2004·Stem Cells·Hanna SegevJoseph Itskovitz-Eldor
Sep 17, 2004·The Journal of Clinical Investigation·Rohit N KulkarniC Ronald Kahn
Sep 28, 2004·Diabetes·Mattias HanssonPalle Serup

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Citations

Dec 12, 2012·Journal of Biomedicine & Biotechnology·Fernando de Sá SilvaCarlos Magno da Costa Maranduba
Oct 23, 2015·Journal of Molecular Endocrinology·Michael WelshBjörn Åkerblom
May 9, 2012·Acta Biochimica Et Biophysica Sinica·Shalmoli BhattacharyyaKishan Lal Khanduja
Jun 13, 2008·Clinical Science·Cecilia Annerén

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