Shc and Enigma are both required for mitogenic signaling by Ret/ptc2.

Molecular and Cellular Biology
K DurickS S Taylor

Abstract

Ret/ptc2 is a constitutively active, oncogenic form of the c-Ret receptor tyrosine kinase. Like the other papillary thyroid carcinoma forms of Ret, Ret/ptc2 is activated through fusion of the Ret tyrosine kinase domain to the dimerization domain of another protein. Investigation of requirements for Ret/ptc2 mitogenic activity, using coexpression with dominant negative forms of Ras and Raf, indicated that these proteins are required for mitogenic signaling by Ret/ptc2. Because activation of Ras requires recruitment of Grb2 and SOS to the plasma membrane, the subcellular distribution of Ret/ptc2 was investigated, and it was found to localize to the cell periphery. This localization was mediated by association with Enigma via the Ret/ptc2 sequence containing tyrosine 586. Because Shc interacts with MEN2 forms of Ret, and because phosphorylation of Shc results in Grb2 recruitment and subsequent signaling through Ras and Raf, the potential interaction between Ret/ptc2 and Shc was investigated. The PTB domain of Shc also interacted with Ret/ptc2 at tyrosine 586, and this association resulted in tyrosine phosphorylation of Shc. Coexpression of chimeric proteins demonstrated that mitogenic signaling from Ret/ptc2 required both recruitm...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M A PierottiA Fusco
Jan 1, 1991·Methods in Enzymology·T A KunkelJ McClary
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M P KampsB M Sefton
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Oct 20, 1995·The Journal of Biological Chemistry·K DurickS S Taylor
Jan 1, 1995·Molecular and Cellular Biology·K PumigliaR Jove
Jan 1, 1994·Annual Review of Cell Biology·P van der GeerR A Lindberg
Aug 1, 1994·Human Molecular Genetics·T AttieS Lyonnet
Jun 27, 1996·Nature·P DurbecM Saarma
Jul 4, 1996·Nature·M W MooreA Rosenthal
Jul 4, 1996·Nature·J J TreanorA Rosenthal
May 31, 1996·The Journal of Biological Chemistry·K DurickS S Taylor
Jul 5, 1996·The Journal of Biological Chemistry·R WuG N Gill
Apr 1, 1996·Trends in Genetics : TIG·B PasiniG Romeo
Dec 1, 1996·Trends in Biochemical Sciences·J Saras, C H Heldin
Apr 4, 1997·The Journal of Biological Chemistry·Q TongS M Jhiang

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Citations

Mar 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yunshan LiuLouisa Titus
Jan 22, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jennifer KrcmeryHans-Georg Simon
Aug 15, 2002·Biochemical and Biophysical Research Communications·Maria Grazia BorrelloMarco A Pierotti
Feb 16, 2002·Developmental Biology·Ming-Jer TangGregory R Dressler
Mar 17, 1999·Biochimica Et Biophysica Acta·P W Schenk, B E Snaar-Jagalska
Jun 2, 2007·Developmental Biology·Doyeob Kim, Gregory R Dressler
Jun 5, 1999·Molecular and Cellular Neurosciences·M S AiraksinenM Saarma
Jan 28, 2014·PloS One·Stephen C KalesStanley Lipkowitz
Jan 1, 2010·Journal of Molecular Cell Biology·Ming ZhengJu Chen
Jun 11, 2005·Experimental Cell Research·Romain BarrèsJean-François Tanti
Nov 15, 2001·Advances in Anatomic Pathology·G Tallini, S L Asa
Jun 8, 1999·Molecular Biology of the Cell·P M GuyG N Gill
Oct 13, 2017·Head & Neck·Anthony A FirekSalma Khan
Nov 19, 2005·Journal of Neuroscience Research·Rebecca Hui Kwan LeeSiu Yuen Chan
Nov 10, 2010·The Journal of Clinical Investigation·Cho-Rok JungDong-Soo Im
May 15, 2012·Marine Biotechnology·Mahaut de VareillesIvar Rønnestad
Dec 15, 2000·Oncogene·S M Jhiang
Mar 12, 2002·The Journal of Biological Chemistry·Toshifumi FukudaMasahide Takahashi
May 21, 2003·Journal of Internal Medicine·K KurokawaM Takahashi
Oct 18, 2005·Molecular and Cellular Biology·Adrianne WongVassilis Pachnis
Jul 20, 2006·Endocrine Reviews·Jan Willem B de GrootRobert M W Hofstra
Feb 7, 2001·The Journal of Biological Chemistry·T ValleniusT P Mäkelä
Sep 7, 2001·Cytokine & Growth Factor Reviews·M Takahashi
May 10, 2003·Blood Cells, Molecules & Diseases·George B SegelNancy Wang

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