Shc binding to nerve growth factor receptor is mediated by the phosphotyrosine interaction domain.

The Journal of Biological Chemistry
I DikicB Margolis

Abstract

Shc is an adaptor protein that contains two phosphotyrosine-binding domains, a Src homology 2 (SH2) domain and the newly described phosphotyrosine interaction (PI) domain. Shc interacts with several tyrosine-phosphorylated proteins and is itself tyrosine-phosphorylated in cells stimulated with a variety of growth factors and cytokines. Upon phosphorylation, Shc binds to the Grb2.Sos complex leading to the activation of the Ras signaling pathway. Mutational analysis of the nerve growth factor (NGF) receptor (TrkA) suggested that the binding of Shc to the activated receptor is required for NGF-induced neuronal differentiation of PC12 cells. Here we report that the PI domain of Shc directly binds to tyrosine 490 on the autophosphorylated NGF receptor. The PI domain specifically recognizes an I/LXN-PXpY motif (where p indicates phosphorylation) as determined by phosphopeptide competition assay. In addition, the PI domain is able to efficiently compete for binding of full-length Shc proteins to the NGF receptor. In PC12 cells, the Shc SH2 domain interacts with an unidentified tyrosine-phosphorylated protein of 115 kDa but not with the activated NGF receptor. The ability of Shc to interact with different tyrosine-phosphorylated prote...Continue Reading

References

Sep 1, 1992·Neuron·J Schlessinger, A Ullrich
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Aug 1, 1994·Molecular and Cellular Biology·A G BatzerJ Schlessinger
Dec 16, 1994·Science·W M Kavanaugh, L T Williams
Feb 16, 1995·Nature·T Pawson
Mar 10, 1995·Cell·P Bork, B Margolis
Jan 27, 1995·Cell·G B CohenD Baltimore
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·K S CampbellD C Pallas
Feb 1, 1994·Current Opinion in Genetics & Development·J Schlessinger
Feb 1, 1994·Current Opinion in Genetics & Development·C J Marshall
Aug 1, 1993·Trends in Biochemical Sciences·J Schlessinger

❮ Previous
Next ❯

Citations

Jun 26, 1999·Microscopy Research and Technique·L J Klesse, L F Parada
Nov 18, 1998·Journal of Neuroscience Research·L AibelM V Chao
Feb 19, 2000·Journal of Neuroscience Research·H YanoM V Chao
Sep 29, 1999·Journal of Cellular Biochemistry·Y Xu, G Carpenter
Jan 9, 2001·Journal of Cellular Physiology·C MirandaE Van Obberghen
Jun 4, 1999·Progress in Biophysics and Molecular Biology·S R Hubbard
Nov 26, 1998·Trends in Neurosciences·E Cattaneo, P G Pelicci
Jan 1, 1997·Progress in Neurobiology·M YamadaH Hatanaka
Aug 6, 2002·Molecular and Cellular Endocrinology·R X SongW Yue
Aug 1, 1997·Current Opinion in Chemical Biology·S E Shoelson
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P van der GeerT Pawson
Apr 25, 2009·The Journal of Biological Chemistry·Seong-Moon CheongJin-Kwan Han
Jun 1, 1997·The European Journal of Neuroscience·E M Fitzgerald, A C Dolphin
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H MorenoR Llinás
Feb 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert X SongRichard J Santen
Aug 26, 1999·Trends in Endocrinology and Metabolism : TEM·B Margolis
Jun 20, 2003·The Journal of Physiology·Jennifer K Bonnington, Peter A McNaughton
Jun 28, 2000·The Journal of Cell Biology·U SchaeperW Birchmeier
Oct 28, 2010·Biochemical and Biophysical Research Communications·Eun Joo Jung, Choong Won Kim
Sep 13, 2008·Journal of Neuroimmunology·Anton B TonchevTetsumori Yamashima
Aug 2, 2002·Biochemical and Biophysical Research Communications·Philip E TarrLuciano D'Adamio
Sep 1, 1996·The Journal of Laboratory and Clinical Medicine·B Margolis
Jul 19, 2005·The Journal of Steroid Biochemistry and Molecular Biology·R J SantenL Berstein
Dec 2, 1999·Experimental Cell Research·W J Friedman, L A Greene
Sep 30, 1998·The European Journal of Neuroscience·P GanjuI F James
Aug 22, 2016·Molecular Neurobiology·Neha SamiImtaiyaz Hassan
Oct 5, 2007·Pharmacology & Therapeutics·V Freund-Michel, N Frossard
Apr 3, 1999·The Journal of Biological Chemistry·L RuiC Carter-Su
Feb 16, 2017·Frontiers in Molecular Neuroscience·Nadia CanuPietro Calissano

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