Shc, Grb2, Sos1, and a 150-kilodalton tyrosine-phosphorylated protein form complexes with Fms in hematopoietic cells.

Molecular and Cellular Biology
M N LioubinL R Rohrschneider

Abstract

Fms, the macrophage colony-stimulating factor (M-CSF) receptor, is normally expressed in myeloid cells and initiates signals for both growth and development along the monocyte/macrophage lineage. We have examined Fms signal transduction pathways in the murine myeloid progenitor cell line FDC-P1. M-CSF stimulation of FDC-P1 cells expressing exogenous Fms resulted in tyrosine phosphorylation of a variety of cellular proteins in addition to Fms. M-CSF stimulation also resulted in Fms association with two of these tyrosine-phosphorylated proteins, one of which was identified as the 55-kDa Shc, which is shown in other systems to be involved in growth stimulation, and the other was a previously uncharacterized 150-kDa protein (p150). Fms also formed complexes with Grb2 and Sos1, and neither contained phosphotyrosine. Whereas both Grb2 and Sos1 complexed with Fms only after M-CSF stimulation, the amount of Sos1 complexed with Grb2 was not M-CSF dependent. Shc coimmunoprecipitated Sos1, Grb2, and tyrosine-phosphorylated p150, while Grb2 immunoprecipitates contained mainly phosphorylated p150, Fms, Shc, and Sos1. Shc interacted with tyrosine-phosphorylated p150 via its SH2 domain, and the Grb2 SH2 domain likewise bound tyrosine-phosphor...Continue Reading

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Citations

Jan 1, 1997·Molecular Reproduction and Development·L R RohrschneiderK Carlberg
Oct 6, 2007·Immunologic Research·Masaki KashiwadaPaul B Rothman
Jun 4, 1999·Progress in Biophysics and Molecular Biology·M HuberG Krystal
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Sep 18, 1997·Immunology Today·S TridandapaniK M Coggeshall
Oct 18, 2002·Experimental Hematology·Annette EhrhardtJohn W Schrader
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E DamenG Krystal
Dec 1, 1995·Australian and New Zealand Journal of Medicine·N G DellaD D Bowtell
Mar 24, 2009·Analytical Biochemistry·Yee-Guide Yeung, E Richard Stanley
Feb 16, 2006·Journal of Molecular Biology·William A McLaughlinWei Wang
Nov 29, 2011·Enzyme Research·Qingxiu Zhang, Francois X Claret
Jun 25, 2004·Molecular & Cellular Proteomics : MCP·Kevin WilhelmsenPeter van der Geer
Aug 1, 1995·FEBS Letters·P ChardinA Ducruix
Aug 5, 2000·The Journal of Biological Chemistry·J Clyde-SmithJ F Hancock
Feb 13, 1999·The Journal of Biological Chemistry·G BehreD G Tenen
Dec 10, 1999·The Journal of Biological Chemistry·K L BergE R Stanley

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