Shear acoustic wave biosensor for detecting DNA intrinsic viscosity and conformation: a study with QCM-D

Biosensors & Bioelectronics
Achilleas TsortosElectra Gizeli

Abstract

Direct biosensors are devices operating by monitoring the amount of surface-bound analyte. In this work a new approach is presented where a label-free acoustic biosensor, based on a QCM-D device, and solution viscosity theory, are used to study DNA intrinsic viscosity. The latter is quantitatively related to the DNA conformation and specifically the molecule's shape and size, in a manner that is independent of the amount of bound DNA mass. It is shown that acoustic measurements can clearly distinguish between ds-DNA of same shape (straight rod) but various sizes (from 20 to 198bp (base pairs)) and same mass and size (90bp) but various shapes ("straight", "bent", "triangle"). These results are discussed in the broader context of "coil" and sphere-like molecules detected on surfaces. A mathematical formula is presented relating the length of straight, surface-protruding DNA to the acoustic ratio DeltaD/Deltaf. The development of real-time rapid techniques for the characterization of DNA intrinsic curvature as well as DNA conformational changes upon interaction with proteins is of significance to analytical biotechnology due to the large number of DNA sequences and potential DNA bending proteins involved in genome analysis and dru...Continue Reading

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Citations

Oct 19, 2010·European Biophysics Journal : EBJ·Michael Saitakis, Electra Gizeli
Jan 18, 2012·Analytical Chemistry·George PapadakisElectra Gizeli
Jul 9, 2013·Analytical Chemistry·Víctor Agmo HernándezKatarina Edwards
Oct 10, 2012·ACS Applied Materials & Interfaces·Ivan E IvanovTerri A Camesano
Oct 22, 2014·Nature Communications·Aaron WebsterYuki Sato
Oct 15, 2011·Cellular and Molecular Life Sciences : CMLS·Michael Saitakis, Electra Gizeli
Jan 21, 2010·FEBS Letters·George PapadakisElectra Gizeli
Oct 27, 2009·Trends in Biotechnology·Guilherme N M FerreiraBrigitte Tomé
Sep 26, 2012·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Kun JiaRodica Elena Ionescu
Mar 31, 2012·Journal of Molecular Biology·Robert H WaltersRegina M Murphy
Jul 1, 2015·Analytical Chemistry·Alina OsypovaPierre Labbé
May 28, 2016·Analytical Chemistry·Achilleas TsortosElectra Gizeli
Jun 20, 2013·Scientific Reports·G PapadakisE Gizeli
Jun 24, 2017·Biochimica Et Biophysica Acta. Biomembranes·Ileana F MárquezMarisela Vélez
Jun 23, 2015·Chemical Communications : Chem Comm·Achilleas TsortosElectra Gizeli
Sep 9, 2017·Sensors·Román FernándezAntonio Arnau
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Mar 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Taisuke Kojima
Jun 4, 2011·Biopolymers·Achilleas TsortosElectra Gizeli
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Sep 15, 2009·Biosensors & Bioelectronics·George PapadakisElectra Gizeli
Jun 15, 2011·Biosensors & Bioelectronics·Konstantinos Mitsakakis, Electra Gizeli

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