Shear stress-induced binding of von Willebrand factor to platelets

Biorheology
K KonstantopoulosJ L Moake

Abstract

Shear stress-induced platelet aggregation requires von Willebrand factor (vWF), platelet glycoprotein (GP) Ib, GPIIb-IIIa, Ca2+, and adenosine diphosphate (ADP). Recent reports using vWF labeled with either 125I or fluorescein isothiocyanate (FITC) have demonstrated that in shear-fields, vWF binds to both GPIb and GPIIb-IIIa. The sequence of the vWF finding to the two platelet receptors has not been precisely determined in these reports. In this study, a flow cytometry technique using a primary anti-vWF antibody and a secondary FITC IgG antibody was used to measure shear stress-induced vWF binding to platelets. Washed normal platelets suspended at 50,000/microliters with purified large vWF multimers were exposed to laminar shear stresses of 15 to 120 dynes/cm2 for 30 sec. At this low platelet count, little or no aggregation occurred in the shear fields. A significant increase in post-shear vWF-positive platelets was consistently observed. Experiments with platelets from normal and severe von Willebrand's disease (vWD) (which lack plasma and platelet alpha-granule vWF) demonstrated that exogenous vWF predominately contributed to the platelet-vWF binding. Blockade of platelet GPIb with the monoclonal anti-GPIb antibody, 6D1, comp...Continue Reading

References

Jan 1, 1977·Journal of Biomechanics·L D BackD W Crawford
Apr 1, 1991·The Journal of Clinical Investigation·Y IkedaI Itagaki
Sep 1, 1986·Biophysical Journal·T K Belval, J D Hellums
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S TimmonsJ Hawiger
Jul 1, 1983·The Journal of Clinical Investigation·Z M RuggeriR R Montgomery
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K M BrinkhousR H Wagner
Aug 15, 1984·Thrombosis Research·L Thorell, B Blombäck
Nov 1, 1984·Microvascular Research·T BelvalR T Solis
Nov 1, 1994·Annals of Biomedical Engineering·S M SlackV T Turitto

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Citations

Mar 31, 2012·Annals of Biomedical Engineering·Mathew H Flamm, S L Diamond
May 29, 2002·Thrombosis Research·Tze-Wen ChungShu-Hsun Chu
Jun 6, 2000·Advanced Drug Delivery Reviews·K KonstantopoulosL V McIntire
Aug 23, 2002·The New England Journal of Medicine·Joel L Moake
Jan 31, 2009·Kidney International. Supplement·Han-Mou Tsai
Aug 15, 1998·The American Journal of the Medical Sciences·J L Moake, T W Chow
Jul 31, 2007·Progress in Retinal and Eye Research·Renata PiccianiSanjoy K Bhattacharya
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·Y OzakiM C Berndt
Oct 10, 2012·Transfusion·Robbie K MontgomeryAnand K Ramasubramanian
Nov 10, 2004·Biophysical Journal·Nipa A ModyMichael R King
Feb 6, 2016·Journal of Thrombosis and Haemostasis : JTH·Lawrence F Brass, Scott L Diamond
Jan 31, 2002·Annual Review of Medicine·Joel L Moake
Nov 7, 2015·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Gungor Cagdas Dincel, Hasan Tarik Atmaca
Mar 31, 2017·Platelets·Jevgenia Zilberman-RudenkoOwen J T McCarty
Aug 27, 2013·Physical Biology·Jennifer K W Chesnutt, Hai-Chao Han
Nov 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Xinkang WangGiora Z Feuerstein
Apr 3, 2001·The Journal of Biological Chemistry·G SoslauZ M Ruggeri

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