PMID: 9541241Apr 16, 1998Paper

Sheath proteins: synthesis, secretion, degradation and fate in forming enamel

European Journal of Oral Sciences
T UchidaO Takahashi

Abstract

We investigated expression of ameloblastin and sheathlin, recently cloned enamel matrix proteins from the rat and pig, in forming enamel immunocytochemically and immunochemically, using region-specific antibodies. The results obtained from the rat and pig were essentially the same. Antibodies which recognize the N-terminal region stained the secretory machinery of the secretory ameloblast and the entire thickness of the enamel matrix, especially the peripheral region of the enamel rod. Immunostained protein bands were observed near 65 or 70 kDa and below 20 kDa. C-terminal-specific antibodies stained the secretory machinery of the ameloblast and the immature enamel adjacent to the secretion sites. Immunostained protein bands were found ranging from 25 to 70 kDa. Antibodies which recognize a region in the protein just prior to the C-terminal region stained the cis-side of the Golgi apparatus but not the enamel matrix. Immunostained protein bands were observed of about 55 kDa. These results suggest that post-translational and post-secretory modifications of ameloblastin and sheathlin are similar to each other, and further showed that their cleaved N-terminal polypeptides concentrate in the prism sheath. We propose that sheathlin ...Continue Reading

Citations

May 16, 2014·Marine Drugs·David W GreenHan-Sung Jung
May 13, 2006·Calcified Tissue International·Y NakamuraS P Lyngstadaas
May 11, 2005·Journal of Oral Science·María Angélica Torres-QuintanaNelson Lobos
Sep 22, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·M Zeichner-David
May 9, 2009·Journal of Anatomy·Javier Catón, Abigail S Tucker
Jan 25, 2012·European Journal of Oral Sciences·Yasuo YamakoshiJames P Simmer
Sep 24, 2004·Biochemical and Biophysical Research Communications·Hanumanth H RavindranathRajeswari M H Ravindranath
Aug 12, 1999·Journal of Structural Biology·A G FinchamJ P Simmer
Aug 15, 2014·Frontiers in Physiology·Parichita MazumderJanet Moradian-Oldak
Jan 14, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J D Bartlett, J P Simmer
Feb 28, 2002·Gene·Seikou ShintaniTakashi Ooshima
Sep 25, 2007·Journal of Dental Research·K KobayashiJ P Simmer
Oct 13, 2004·Calcified Tissue International·Y NakamuraS P Lyngstadaas
Jan 26, 2007·Journal of Dental Research·T IwataJ P Simmer
Oct 12, 2005·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Seikou ShintaniTakashi Ooshima
Nov 11, 2011·Tissue Engineering. Part B, Reviews·H Michelle GrandinMichel Dard
May 6, 2006·European Journal of Oral Sciences·Yasuo YamakoshiJames P Simmer
May 6, 2006·European Journal of Oral Sciences·Steven J BrookesJennifer Kirkham
Dec 23, 2006·European Journal of Oral Sciences·Axel SpahrGita Pezeshki
Aug 12, 2017·Frontiers in Physiology·Øystein StakkestadJanne E Reseland
Aug 22, 2017·Frontiers in Physiology·Cong-Dat PhamYong-Hee P Chun
May 2, 2001·European Journal of Oral Sciences·C K MårdhK Forsman-Semb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.