Shed human enterocytes as a tool for the study of expression and function of intestinal drug-metabolizing enzymes and transporters

Clinical Pharmacology and Therapeutics
Hartmut GlaeserMartin F Fromm

Abstract

Intestinal metabolism and transport are now recognized as protective barriers against orally ingested xenobiotics, including drugs. However, in vitro studies of the expression and function of intestinal proteins are hampered by the limited availability of human intestinal tissues. Because enterocytes are constantly shed in large numbers into the gut lumen, this study investigated whether these cells could be collected with a multilumen perfusion catheter and whether they are functionally active. In healthy volunteers, a 20-cm isolated jejunal segment was generated with the perfusion catheter by inflating 2 balloons with air. Shed cells were characterized by fluorescence-activated cell sorting analysis for leukocyte-specific CD45 and enterocyte-specific villin, as well as for apoptosis. Homogenates of the cells were used for reverse transcriptase polymerase chain reaction and Western blotting. Cytochrome P450 enzyme activity was determined with the calcium channel blocker verapamil as a substrate. On average, 4.83 mg protein and 56.23 million cells were collected from a 20-cm segment during 2 hours. A total of 84.2% of the cells were positive for enterocyte-specific villin, and only 1.6% of the collected cells were positive for ...Continue Reading

Citations

Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Frank J Gonzalez, Ai-Ming Yu
Jul 16, 2008·Expert Opinion on Drug Metabolism & Toxicology·Aleksandra GaletinJ Brian Houston
Jan 29, 2005·British Journal of Clinical Pharmacology·H GlaeserM F Fromm
Jun 11, 2003·European Journal of Clinical Investigation·M LindellM A Lang
Dec 16, 2016·Biopharmaceutics & Drug Disposition·Jean-Marie NicolasAnna-Lena Ungell
Jul 29, 2003·Clinical Pharmacokinetics·Mikko NiemiKari T Kivistö
Jan 12, 2007·Clinical Pharmacology and Therapeutics·H GlaeserR B Kim
Mar 9, 2010·Drug Metabolism and Pharmacokinetics·Aleksandra GaletinJ Brian Houston
Jun 10, 2006·The Journal of Pharmacology and Experimental Therapeutics·Aleksandra Galetin, J Brian Houston
Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Rajinder K BhardwajMartin F Fromm
Oct 4, 2007·The Journal of Pharmacy and Pharmacology·Urban Fagerholm
Sep 16, 2003·Pharmacogenetics·Florian LäppleKari T Kivistö
May 21, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Niclas PetriGary Williamson
Nov 20, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·K Sandy Pang
Apr 18, 2020·Gastroenterology·Rasmus IversenLudvig M Sollid

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