Shedding of Escherichia coli O157:H7 in dairy cattle housed in a confined environment following waterborne inoculation.

Applied and Environmental Microbiology
J ShereD M Schaefer

Abstract

A study of Escherichia coli O157:H7 transmission and shedding was conducted with bull calves housed in individual pens within a confined environment. For comparative purposes, the numbers and duration of E. coli O157:H7 shedding in naturally infected calves were monitored after a single purchased calf (calf 156) tested positive prior to inoculation. During the next 8 days, the calves in adjacent pens and a pen directly across a walkway from calf 156 began to shed this serotype O157:H7 strain. Five of the eight calves in this room shed this O157:H7 strain at some time during the following 8 weeks. The numbers of E. coli O157:H7 isolates shed in these calves varied from 60 to 10(5) CFU/g of feces, and the duration of shedding ranged from 17 to >31 days. The genomic DNAs from isolates recovered from these calves were indistinguishable when compared by using XbaI digestion and pulsed-field gel electrophoresis. Inoculation of calves with 1 liter of water containing ca. 10(3) to 10(4) CFU of E. coli O157:H7/ml resulted in shedding in 10 of 12 calves (trial 1, 4 of 4 calves; trial 2, 6 of 8 calves). The inoculated calves shed the inoculation strain (FRIK 1275) as early as 24 h after administration. The duration of shedding varied from...Continue Reading

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Citations

Dec 2, 2010·Foodborne Pathogens and Disease·Witold A Ferens, Carolyn J Hovde
Feb 22, 2011·Applied and Environmental Microbiology·Kwang Cheol JeongCharles W Kaspar
Mar 4, 2008·Applied and Environmental Microbiology·Nancy A Cornick, Hung Vukhac
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Oct 3, 2018·Journal of Environmental Quality·Gurleen TaggarMoussa Sory Diarra
Jan 7, 2005·Journal of Food Protection·P McGeeN Leonard

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