Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei

Scientific Reports
Carolina Agop-NersesianVolker T Heussler

Abstract

The hepatic stage of the malaria parasite Plasmodium is accompanied by an autophagy-mediated host response directly targeting the parasitophorous vacuolar membrane (PVM) harbouring the parasite. Removal of the PVM-associated autophagic proteins such as ubiquitin, p62, and LC3 correlates with parasite survival. Yet, it is unclear how Plasmodium avoids the deleterious effects of selective autophagy. Here we show that parasites trap host autophagic factors in the tubovesicular network (TVN), an expansion of the PVM into the host cytoplasm. In proliferating parasites, PVM-associated LC3 becomes immediately redirected into the TVN, where it accumulates distally from the parasite's replicative centre. Finally, the host factors are shed as vesicles into the host cytoplasm. This strategy may enable the parasite to balance the benefits of the enhanced host catabolic activity with the risk of being eliminated by the cell's cytosolic immune defence.

References

May 1, 1986·The Journal of Infectious Diseases·J Calderón, E E Avila
Jan 1, 1980·The Journal of Experimental Medicine·J CalderónH M Acosta
Sep 14, 2002·Infection and Immunity·Walter BerónMaria I Colombo
Dec 4, 2004·Science·Michinaga OgawaChihiro Sasakawa
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ann-Kristin MuellerStefan H I Kappe
Mar 12, 2005·Cellular Microbiology·Patrícia LeiriãoMaria M Mota
Oct 22, 2005·Molecular Microbiology·Claudia van de SandVolker T Heussler
Mar 22, 2006·Nature Biotechnology·Nadya G GurskayaKonstantin A Lukyanov
Mar 27, 2007·Cellular Microbiology·Daniel E Voth, Robert A Heinzen
Jun 1, 2007·International Journal for Parasitology·Nazneen BanoIsabelle Coppens
Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Jul 21, 2007·Nature Protocols·Sabine ThibergeRogerio Amino
Nov 17, 2007·Cell Host & Microbe·Anthony OrvedahlBeth Levine
Nov 26, 2008·The Journal of Biological Chemistry·Yubao WangAmos Orlofsky
May 15, 2009·Cellular Microbiology·Tomoko IshinoPatricia Baldacci
Sep 15, 2009·Nature Cell Biology·Yuko YoshikawaChihiro Sasakawa
Oct 9, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yiyu T ZhengJohn H Brumell
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons
Dec 15, 2011·Nature Reviews. Molecular Cell Biology·Patrice CodognoTassula Proikas-Cezanne
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Feb 8, 2013·Autophagy·Nina EickelVolker T Heussler
Mar 31, 2015·Eukaryotic Cell·Joachim M MatzTaco W A Kooij
Sep 25, 2015·Cellular Microbiology·Carolina Thieleke-MatosMiguel C Seabra
May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rachasak BoonhokMarisa Ponpuak
Sep 3, 2016·FEMS Microbiology Reviews·Emma S Sherling, Christiaan van Ooij
Nov 29, 2016·Nature Reviews. Microbiology·Mariana De NizVolker T Heussler
Jan 27, 2017·Molecular Biology of the Cell·Wiebke PetersenAlyssa Ingmundson

❮ Previous
Next ❯

Citations

Mar 13, 2018·FEMS Microbiology Reviews·Carolina Agop-NersesianVolker Theo Heussler
Aug 26, 2017·PloS One·Jacqueline Schmuckli-MaurerVolker Theo Heussler
Mar 10, 2020·Cellular Microbiology·Mariana De NizCarolina Agop-Nersesian
Jan 9, 2020·Malaria Journal·Benjamin WinerVincent R Gerbasi
Sep 19, 2020·Cell & Bioscience·George Ghartey-KwansahJohnson Nyarko Boampong
Oct 3, 2018·Frontiers in Cell and Developmental Biology·Robert J EvansEva-Maria Frickel
Feb 17, 2021·Trends in Parasitology·Silvia A ArredondoStefan H I Kappe
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Annemarie Voorberg-van der WelAnne-Marie Zeeman
May 8, 2021·Frontiers in Cell and Developmental Biology·Kerry WoodsPhilipp Olias
May 21, 2021·Journal of Cell Science·Mariana De NizCarolina Agop-Nersesian
Jul 2, 2021·Frontiers in Cellular and Infection Microbiology·Kayla SylvesterEmily R Derbyshire

❮ Previous
Next ❯

Methods Mentioned

BETA
lipidation
Fluorescence Recovery After Photobleaching
Fluorescence
PCR
Infection
transgenic
Transfection
transfections
fluorescence microscopy

Software Mentioned

Hyugens deconvolution
Fiji
SVI
Huygens deconvolution
StackReg Plugin

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.