Shedding of sabin poliovirus Type 3 containing the nucleotide 472 uracil-to-cytosine point mutation after administration of oral poliovirus vaccine

The Journal of Infectious Diseases
Claudia V MartinezYvonne Maldonado

Abstract

A uracil-to-cytosine point mutation at nucleotide (nt) 472 of Sabin oral poliovirus vaccine (OPV) type 3 is found in conjunction with vaccine-associated paralytic poliomyelitis (VAPP). Direct RNA extraction and mutant analysis by polymerase chain reaction and restriction enzyme cleavage were used to identify this point mutation in clinical samples. A total of 238 stool samples were obtained from 28 healthy infants for 6 weeks after OPV vaccination. More than 25% of infants shed OPV3 in the week after vaccination, with a decrease on day 6. A second wave of OPV3 shedding occurred beginning the second week after vaccination and was maintained through the end of the study period. During the first week after vaccination, the proportion of nt 472 mutants in the shed OPV3 increased from undetectable to almost 100%. During the second shedding period, the proportion of nt 472 mutants remained close to 100%. These results suggest that selective mutation drives the VAPP-associated nt 472 point mutation for OPV3 in the human gastrointestinal tract.

Citations

Sep 8, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V PliakaP Markoulatos
Jul 29, 2011·Journal of Virology·Karen Z Lancaster, Julie K Pfeiffer
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Mar 11, 2008·FEMS Immunology and Medical Microbiology·Evaggelos DedepsidisPanayotis Markoulatos
Oct 31, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan AltamiranoYvonne Maldonado
Aug 4, 2018·MSystems·Matthew L PaffClaus O Wilke
Oct 31, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Meira S HalpernYvonne Maldonado

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