Sheep Placenta Cotyledons: A Noninvasive Source of Ovine Mesenchymal Stem Cells

Tissue Engineering. Part C, Methods
Iris RibitschFlorien Jenner

Abstract

Sheep are one of the most frequently used large animal models in stem cell research. However, minimal invasive or noninvasive sources of mesenchymal stem cells (MSCs) in sheep are scarce. In the light of the principles of the 3Rs (reduce, refine, replace), it would therefore be desirable to identify a minimally invasive or noninvasive ovine MSC source. In humans, the chorionic villi of the placenta, which can be noninvasively harvested as part of the afterbirth, have been identified as a rich source of MSCs. Therefore, in the present study, ovine placenta cotyledons, which have similar function and structure to human chorionic villi, were tested for their potential use as a noninvasive source of ovine MSCs. Through mincing of the placental cotyledons, collagenase digestion, and Ficoll density gradient centrifugation, combined with plastic adherence selection, MSCs were successfully isolated. Their morphological, immunophenotypical, and cellular growth characteristics, as well as their proliferation, differentiation, and migration potential, were evaluated and compared to the currently best-researched MSC source, bone marrow-derived stem cells. Ovine cotyledons were shown to be a reliable, abundant source for the noninvasive, pa...Continue Reading

References

Jun 10, 2000·British Journal of Haematology·A EricesJ J Minguell
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Dec 3, 2003·Fetal Diagnosis and Therapy·Christopher D PoradaEsmail D Zanjani
Mar 17, 2004·Circulation·Judith A AireyEsmail D Zanjani
Mar 24, 2004·Cell·Elaine FuchsGeraldine Guasch
Jul 14, 2004·Yonsei Medical Journal·Graca Almeida PoradaEsmail D Zanjani
Dec 8, 2004·Stem Cells·Pieternella S In 't AnkerHumphrey H H Kanhai
Dec 8, 2004·Best Practice & Research. Clinical Obstetrics & Gynaecology·Ian Rogers, Robert F Casper
Jan 13, 2006·Biochemical and Biophysical Research Communications·Xiaohong ZhangTsuneo A Takahashi
May 2, 2006·Stem Cells and Development·Shaun M KunisakiDario O Fauza
Jul 1, 2006·Nature·David T Scadden
Aug 17, 2006·BMC Musculoskeletal Disorders·Katja M R NussBrigitte von Rechenberg
Jan 9, 2007·Nature Biotechnology·Paolo De CoppiAnthony Atala
Jun 1, 2007·BMC Biotechnology·Thomas G KochDean H Betts
Aug 19, 2007·Differentiation; Research in Biological Diversity·Samoa GiovanniniDobrila Nesic
Aug 28, 2007·Biochemical and Biophysical Research Communications·Steven M HoynowskiKathy E Mitchell
Nov 21, 2007·Cell Proliferation·L PerinR E De Filippo
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·D Leanne Jones, Amy J Wagers
Dec 25, 2007·Neuromuscular Disorders : NMD·Mariane SeccoMayana Zatz
Jan 16, 2008·Journal of Cellular and Molecular Medicine·Akiva J Marcus, Dale Woodbury
Apr 30, 2008·Methods in Cell Biology·Kiran SeshareddyMark L Weiss
Jun 21, 2008·Stem Cells·Lindolfo da Silva MeirellesNance Beyer Nardi
Sep 13, 2008·Cell Stem Cell·Mihaela CrisanBruno Péault
Oct 14, 2008·Tissue Engineering. Part a·Lindolfo da Silva MeirellesArnold I Caplan
Nov 11, 2008·Cell Biology International·Simona PasseriAlessandra Coli
Nov 14, 2008·Stem Cells and Development·Sarah BarlowKerry Atkinson
Nov 19, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·M R ZarrinkalamR J Moore
Dec 31, 2008·Journal of Cellular Physiology·Rosa C McCartyCory J Xian
Jul 28, 2009·In Vitro Cellular & Developmental Biology. Animal·Xiuqing Wang, Daphne Moutsoglou
Jan 26, 2010·Equine Veterinary Journal·S BartholomewL D Galuppo
Mar 12, 2010·Journal of Cellular and Molecular Medicine·Tiziana MontemurroLorenza Lazzari

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Citations

Oct 17, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Janna L MorrisonRoss L Tellam
Jan 20, 2020·Analytical Biochemistry·M A El-SamahyFeng Wang
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Ejaz R DarTalha Bin Emran

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Methods Mentioned

BETA
cesarean sections
dissection
density gradient filtration
biopsy
FACS
Flow Cytometry

Software Mentioned

SPSS

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