Sheep-Specific Immunohistochemical Panel for the Evaluation of Regenerative and Inflammatory Processes in Tissue-Engineered Heart Valves

Frontiers in Cardiovascular Medicine
Sylvia DekkerAnthal I P M Smits

Abstract

The creation of living heart valve replacements via tissue engineering is actively being pursued by many research groups. Numerous strategies have been described, aimed either at culturing autologous living valves in a bioreactor (in vitro) or inducing endogenous regeneration by the host via resorbable scaffolds (in situ). Whereas a lot of effort is being invested in the optimization of heart valve scaffold parameters and culturing conditions, the pathophysiological in vivo remodeling processes to which tissue-engineered heart valves are subjected upon implantation have been largely under-investigated. This is partly due to the unavailability of suitable immunohistochemical tools specific to sheep, which serves as the gold standard animal model in translational research on heart valve replacements. Therefore, the goal of this study was to comprise and validate a comprehensive sheep-specific panel of antibodies for the immunohistochemical analysis of tissue-engineered heart valve explants. For the selection of our panel we took inspiration from previous histopathological studies describing the morphology, extracellular matrix composition and cellular composition of native human heart valves throughout development and adult stage...Continue Reading

References

Feb 10, 1995·The Journal of Biological Chemistry·E SchönherrH Kresse
Dec 1, 1995·The Annals of Thoracic Surgery·T ShinokaJ E Mayer
Aug 1, 1996·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·M L AliC M Duran
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·X LiuR Jaenisch
Nov 18, 2000·Circulation·S P HoerstrupJ E Mayer
Jan 24, 2002·Nature·Tomoyuki NakamuraKenneth R Chien
May 9, 2003·The Annals of Thoracic Surgery·Rainer G LeyhHeike Mertsching
Sep 17, 2003·Glycoconjugate Journal·Charles C Reed, Renato V Iozzo
Jul 13, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·K Schenke-LaylandU A Stock
Oct 1, 2004·The Journal of Thoracic and Cardiovascular Surgery·Elena Rabkin-AikawaFrederick J Schoen
Dec 18, 2004·Biomaterials·Anita MolCarlijn V C Bouten
Oct 21, 2006·Annals of Biomedical Engineering·Karen Mendelson, Frederick J Schoen
Jul 31, 2007·Veterinary Immunology and Immunopathology·Philip J GriebelWilliam C Davis
Sep 8, 2007·European Journal of Immunology·Jörg HamannMark J Kwakkenbos
Jan 30, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Jessica E Wagenseil, Robert P Mecham
Nov 28, 2008·Molecular Biology of the Cell·Laetitia SabatierDieter P Reinhardt
Apr 1, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Russell A NorrisRichard L Goodwin
May 8, 2009·Expert Review of Medical Devices·Anita MolFrank P T Baaijens
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masahito HoriguchiTomoyuki Nakamura
Jan 22, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Geofrey De VisscherWillem Flameng
Mar 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jason D RohChristopher K Breuer
Jun 12, 2010·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Elizabeth H StephensK Jane Grande-Allen
May 7, 2011·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Jon M CarthyBruce M McManus
May 21, 2011·Nature Reviews. Immunology·Ronald A ReilkoffErica L Herzog
Jul 27, 2011·Journal of Cardiovascular Translational Research·Joyce Bischoff, Elena Aikawa
Jul 28, 2011·International Journal of Inflammation·Gretchen J Mahler, Jonathan T Butcher
Aug 13, 2011·Cellular and Molecular Life Sciences : CMLS·Akira Kudo
Oct 30, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Gretchen J MahlerJonathan T Butcher
May 3, 2013·Development·Miriam VottelerKatja Schenke-Layland
Dec 18, 2013·Biomaterials·Svenja HindererKatja Schenke-Layland

❮ Previous
Next ❯

Citations

Jun 25, 2019·Frontiers in Cardiovascular Medicine·Benjamin L ZhangFrederick J Schoen
Jul 30, 2020·Tissue Engineering. Part a·Prashanth RavishankarKartik Balachandran
Jun 26, 2020·Cell and Tissue Banking·Jan BurkertJaroslav Špatenka
Jun 4, 2021·Acta Biomaterialia·Marcus GroundJillian Cornish

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
flow cytometry

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.

Related Papers

Journal of Cardiovascular Translational Research
M K Sewell-LoftinW David Merryman
The Annals of Thoracic Surgery
Ronald C Elkins
Journal of the American College of Cardiology
Michael Mack
© 2022 Meta ULC. All rights reserved