Sheet of osteoblastic cells combined with platelet-rich fibrin improves the formation of bone in critical-size calvarial defects in rabbits

The British Journal of Oral & Maxillofacial Surgery
Zhifa WangBin Liu

Abstract

Techniques that use sheets of cells have been successfully used in various types of tissue regeneration, and platelet-rich fibrin (PRF) can be used as a source of growth factors to promote angiogenesis. We have investigated the effects of the combination of PRF and sheets of mesenchymal stem cells (MSC) from bone marrow on the restoration of bone in critical-size calvarial defects in rabbits to find out whether the combination promotes bony healing. Sheets of MSC and PRF were prepared from the same donor. We then implanted the combined MSC and PRF in critical-size calvarial defects in rabbits and assessed bony restoration by microcomputed tomography (microCT) and histological analysis. The results showed that PRF significantly increased bony regeneration at 8 weeks after implantation of sheets of MSC and PRF compared with sheets of MSC alone (p=0.0048). Our results indicate that the combination of sheets of MSC and PRF increases bone regeneration in critical-size calvarial defects in rabbits, and provides a new way to improve skeletal healing.

References

Dec 23, 2004·Surgery·Shyh-Jou Shieh, Joseph P Vacanti
Mar 1, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·David M DohanBruno Gogly
Jul 24, 2007·Biomaterials·Ranieri CanceddaMaddalena Mastrogiacomo
Feb 20, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rasmus LundquistMagnus S Agren
Jan 20, 2010·Journal of Tissue Engineering and Regenerative Medicine·Manabu AkahaneYasuhito Tanaka
Nov 26, 2010·Stem Cells and Development·Dongyang MaLiling Ren
Jun 15, 2012·Biomaterials·Fa-Ming ChenQing Yu
Mar 26, 2013·Biochemical and Biophysical Research Communications·Wenxin GengFulin Chen
Nov 19, 2013·International Journal of Oral and Maxillofacial Surgery·H KokemüllerN-C Gellrich
Aug 20, 2014·Tissue Engineering. Part a·Gregor BuehrerAndreas Arkudas
Oct 21, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Zhifa WangBin Liu

❮ Previous
Next ❯

Citations

Jul 19, 2018·Tissue Engineering. Part a·Brianna M RouxEric M Brey
Mar 6, 2019·Journal of Applied Biomaterials & Functional Materials·Hamid TebyanianNafiseh Baheiraei
Jan 10, 2019·Journal of Clinical Periodontology·Nikos DonosElena Calciolari
Jul 9, 2020·Tissue Engineering. Part B, Reviews·Maximilian M MengerTina Histing
Nov 7, 2019·Animals : an Open Access Journal From MDPI·Mathieu RaillardNikola Saulacic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.