Sheets of human retinal progenitor transplants improve vision in rats with severe retinal degeneration

Experimental Eye Research
Bin LinMagdalene J Seiler

Abstract

Loss of photoreceptors and other retinal cells is a common endpoint in retinal degenerate (RD) diseases that cause blindness. Retinal transplantation is a potential therapy to replace damaged retinal cells and improve vision. In this study, we examined the development of human fetal retinal sheets with or without their retinal pigment epithelium (RPE) transplanted to immunodeficient retinal degenerate rho S334ter-3 rats. Sheets were dissected from fetal human eyes (11-15.7 weeks gestation) and then transplanted to the subretinal space of 24-31 d old RD nude rats. Every month post surgery, eyes were imaged by high-resolution spectral-domain optical coherence tomography (SD-OCT). SD-OCT showed that transplants were placed into the subretinal space and developed laminated areas or rosettes, with clear development of plexiform layers first seen in OCT at 3 months post surgery. Several months later, as could be expected by the much slower development of human cells compared to rat cells, transplant photoreceptors developed inner and later outer segments. Retinal sections were analyzed by immunohistochemistry for human and retinal markers and confirmed the formation of several retinal subtypes within the retinal layers. Transplant ce...Continue Reading

Citations

Jun 13, 2019·Seminars in Ophthalmology·David Terrell, Jason Comander
Jun 19, 2019·Stem Cells and Development·Ratnesh K SinghIgor O Nasonkin
Jan 8, 2020·Journal of Tissue Engineering and Regenerative Medicine·Ratnesh K SinghIgor O Nasonkin
Sep 19, 2020·Investigative Ophthalmology & Visual Science·Bin LinMagdalene J Seiler
Nov 2, 2019·Current Ophthalmology Reports·Afnan M Aladdad, Karl E Kador
Nov 3, 2020·Frontiers in Cellular Neuroscience·Ratnesh K Singh, Igor O Nasonkin
Mar 27, 2021·Stem Cell Reports·Suguru YamasakiMichiko Mandai

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