Abstract
Rickettsia conorii and Rickettsia massiliae-Bar29 are related to Mediterranean spotted fever (MSF). They are intracellular microorganisms. The Shell-vial culture assay (SV) improved Rickettsia culture but it still has some limitations: blood usually contains low amount of microorganisms and the samples that contain the highest amount of them are non-sterile. The objectives of this study were to optimize SV culture conditions and monitoring methods and to establish antibiotic concentrations useful for non-sterile samples. 12 SVs were inoculated with each microorganism, incubated at different temperatures and monitored by classical methods and real-time PCR. R. conorii was detected by all methods at all temperatures since 7th day of incubation. R. massiliae-Bar29 was firstly observed at 28°C. Real-time PCR allowed to detected it 2-7 days earlier (depend on temperature) than classical methods. Antibiotics concentration needed for the isolation of these Rickettsia species from non-sterile samples was determined inoculating SV with R. conorii, R. massiliae-Bar29, biopsy or tick, incubating them with different dilutions of antibiotics and monitoring them weekly. To sum up, if a MSF diagnosis is suspected, SV should be incubated at bo...Continue Reading
References
Jun 1, 1989·The Journal of Infectious Diseases·E Espejo-Arenas, D Raoult
Oct 1, 1993·International Journal of Systematic Bacteriology·L Beati, D Raoult
Jul 16, 2003·Annals of the New York Academy of Sciences·G VestrisD Raoult
Mar 5, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christopher D PaddockChristopher A Ohl
Mar 8, 2005·Research in Microbiology·Clarisse RoveryDidier Raoult
May 20, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A PsaroulakiY Tselentis
Aug 30, 2005·The Journal of Infection·M Bernabeu-WittelJ Pachón
Feb 8, 2006·Applied and Environmental Microbiology·Maurício C HortaTeresinha T S Schumaker
Feb 17, 2006·Annals of the New York Academy of Sciences·Tsuneo Uchiyama
Apr 26, 2006·Emerging Infectious Diseases·Giustina VitaleDidier Raoult
Jul 19, 2006·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N CardeñosaI Sanfeliu
Aug 4, 2006·Applied and Environmental Microbiology·Marina E EremeevaGregory A Dasch
Nov 11, 2008·PloS One·Patricia RenestoDidier Raoult
Feb 5, 2011·The American Journal of Tropical Medicine and Hygiene·Emily BeelerMarina E Eremeeva
Jul 21, 2011·Ticks and Tick-borne Diseases·Natacha MilhanoRita de Sousa
Oct 5, 2013·Clinical Microbiology Reviews·Philippe ParolaDidier Raoult
Aug 3, 2014·Parasites & Vectors·Ferran SeguraMaría-Mercedes Nogueras