Shelph2, a bacterial-like phosphatase of the malaria parasite Plasmodium falciparum, is dispensable during asexual blood stage

PloS One
Alexandra MiliuMauld H Lamarque

Abstract

During the erythrocytic cycle of the malaria parasite Plasmodium falciparum, egress and invasion are essential steps finely controlled by reversible phosphorylation. In contrast to the growing number of kinases identified as key regulators, phosphatases have been poorly studied, and calcineurin is the only one identified so far to play a role in invasion. PfShelph2, a bacterial-like phosphatase, is a promising candidate to participate in the invasion process, as it was reported to be expressed late during the asexual blood stage and to reside within an apical compartment, yet distinct from rhoptry bulb, micronemes, or dense granules. It was also proposed to play a role in the control of the red blood cell membrane deformability at the end of the invasion process. However, genetic studies are still lacking to support this hypothesis. Here, we take advantage of the CRISPR-Cas9 technology to tag shelph2 genomic locus while retaining its endogenous regulatory regions. This new strain allows us to follow the endogenous PfShelph2 protein expression and location during asexual blood stages. We show that PfShelph2 apical location is also distinct from the rhoptry neck or exonemes. We further demonstrate PfShelph2 dispensability during ...Continue Reading

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Citations

Aug 1, 2019·Briefings in Functional Genomics·Marcus C S LeeManuel Llinás
Oct 11, 2020·Trends in Parasitology·Jamal KhalifeChristine Pierrot

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Methods Mentioned

BETA
transfection
PCR
transfect
genotyping
PCRs
ChIP-seq
FACS
Fluorescence microscopy

Software Mentioned

LightCycler 480
FACS Diva
Zen Blue edition

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