Shelterin promotes tethering of late replication origins to telomeres for replication-timing control

The EMBO Journal
Shiho OgawaHisao Masukata

Abstract

DNA replication initiates at many discrete loci on eukaryotic chromosomes, and individual replication origins are regulated under a spatiotemporal program. However, the underlying mechanisms of this regulation remain largely unknown. In the fission yeast Schizosaccharomyces pombe, the telomere-binding protein Taz1, ortholog of human TRF1/TRF2, regulates a subset of late replication origins by binding to the telomere-like sequence near the origins. Here, we showed using a lacO/LacI-GFP system that Taz1-dependent late origins were predominantly localized at the nuclear periphery throughout interphase, and were localized adjacent to the telomeres in the G1/S phase. The peripheral localization that depended on the nuclear membrane protein Bqt4 was not necessary for telomeric association and replication-timing control of the replication origins. Interestingly, the shelterin components Rap1 and Poz1 were required for replication-timing control and telomeric association of Taz1-dependent late origins, and this requirement was bypassed by a minishelterin Tpz1-Taz1 fusion protein. Our results suggest that Taz1 suppresses replication initiation through shelterin-mediated telomeric association of the origins at the onset of S phase.

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jan 1, 1989·The Journal of Cell Biology·H Nakayasu, R Berezney
Dec 22, 1999·Nucleic Acids Research·K G SpinkA Chambers
Mar 27, 2001·The Journal of Cell Biology·P HeunS M Gasser
Dec 26, 2001·The Journal of Biological Chemistry·Lorena TaricaniPaul G Young
May 4, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·David M MacAlpine, Stephen P Bell
Feb 12, 2008·Current Protocols in Molecular Biology·Susan L Forsburg
Jun 7, 2008·Science·Tomoichiro MiyoshiFuyuki Ishikawa
Aug 6, 2008·Annual Review of Genetics·Wilhelm Palm, Titia de Lange
Mar 4, 2010·Journal of Cell Science·Hani EbrahimiShin-ichiro Hiraga
Dec 9, 2010·Cold Spring Harbor Symposia on Quantitative Biology·D M GilbertI Hiratani
Jan 27, 2012·Genes & Development·Motoshi HayanoHisao Masai
Dec 4, 2013·The Journal of Cell Biology·Helder C FerreiraSusan M Gasser
Apr 2, 2014·Cell Reports·Stefano MattarocciDavid Shore
Oct 6, 2015·Nature Structural & Molecular Biology·Yutaka KanohHisao Masai
Nov 22, 2015·Cold Spring Harbor Symposia on Quantitative Biology·Vishnu DileepDavid M Gilbert
Jun 18, 2016·Trends in Genetics : TIG·Adriana Gonzalez-Sandoval, Susan M Gasser
Jun 21, 2016·Cell Reports·Sophie ZaaijerJulia Promisel Cooper
May 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Takeshi MizuguchiShiv I S Grewal

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Citations

Mar 9, 2019·Genes·Dominik Boos, Pedro Ferreira
Sep 4, 2019·Nature Reviews. Molecular Cell Biology·Claire MarchalDavid M Gilbert
Sep 27, 2019·FEBS Letters·Juanita C Limas, Jeanette Gowen Cook
Mar 20, 2019·Genes·Ichiro Hiratani, Saori Takahashi
Apr 2, 2020·Nature Structural & Molecular Biology·Aaron Mendez-BermudezEric Gilson
Jul 28, 2020·Trends in Genetics : TIG·Patroula NathanailidouZoi Lygerou
Nov 11, 2020·Journal of Biochemistry·Sana AlaviHisao Masai
Jul 11, 2020·DNA Repair·Susanna Stroik, Eric A Hendrickson

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