Abstract
Integration position-independent expression of human globin transgenes in transgenic mice requires the presence of regulatory elements from the beta-globin locus control region (LCR) in the transgene construct. However, several recent studies have suggested that, while clearly necessary, such elements are not by themselves sufficient to realize this effect. In the case of the human fetal gamma-globin genes, previous results have indicated that additional regulatory information required for sheltering of gamma-globin transgene expression from position effects may reside downstream from the A gamma gene. To investigate this possibility, we established 17 lines of transgenic mice carrying constructs comprising a micro-LCR (microLCR) element, an A gamma-globin gene fragment, and a variable length of 3' sequence information beyond the A gamma 3' HindIII site. gamma-Globin expression during development was studied in 170 individual F2 progeny from these lines. We find that gamma-globin expression becomes sheltered from position effects when the normally position-sensitive microLCR-A gamma construct is extended by 600 bp beyond the 3' HindIII site to include a previously identified regulatory sequence (the A gamma-globin enhancer), th...Continue Reading
References
Apr 1, 1992·Molecular and Cellular Biology·J A LloydJ B Lingrel
Mar 21, 1991·Nature·N Dillon, F Grosveld
Jan 16, 1992·Nature·G Felsenfeld
Jul 1, 1990·Trends in Genetics : TIG·T M Townes, R R Behringer
Jun 25, 1990·Nucleic Acids Research·P FraserF Grosveld
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T EnverG Stamatoyannopoulos
Mar 1, 1989·Genes & Development·T M RyanR L Brinster
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W C ForresterM Groudine
Mar 23, 1989·Nature·D TalbotD R Greaves
Dec 24, 1987·Cell·F GrosveldG Kollias
Aug 15, 1986·Cell·M H Baron, T Maniatis
Jul 15, 1995·European Journal of Biochemistry·S H Orkin
Jul 28, 1995·Gene·P A NavasQ Li
Nov 1, 1993·Molecular and Cellular Biology·I S ThoreyR G Oshima
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G RobertsonE Whitelaw
Jun 1, 1995·Developmental Biology·K M Pawlik, T M Townes
Oct 11, 1994·Nucleic Acids Research·C BoniferA E Sippel
Sep 1, 1994·Molecular and Cellular Biology·Q Li, J A Stamatoyannopoulos
Feb 27, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F GrosveldA Imam
Dec 1, 1993·Molecular and Cellular Biology·G StamatoyannopoulosQ Li
Jul 1, 1993·Molecular and Cellular Biology·M ReitmanG Felsenfeld
Jan 1, 1993·Genes & Development·P FraserF Grosveld
Apr 1, 1993·Trends in Genetics : TIG·N Dillon, F Grosveld
Mar 1, 1996·Nature Genetics·M A BedellN G Copeland
Apr 1, 1996·Trends in Genetics : TIG·E MilotF Grosveld
Citations
Jan 28, 2016·Genome Biology·Holger HeynManel Esteller
Jul 12, 2005·Peptides·Francois Rouzaud, Vincent J Hearing
Aug 21, 2013·PloS One·Hongqiang SunXiang Yang Zhang
May 31, 2014·FEBS Letters·Rakesh S Laishram
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q LiuJ D Engel
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A McDevittS H Orkin
Apr 14, 2010·Nucleic Acids Research·Qian Bian, Andrew S Belmont
Aug 9, 2001·Prenatal Diagnosis·E T LauM H Tang
May 1, 1999·Human Gene Therapy·D W EmeryG Stamatoyannopoulos
Jul 9, 2011·Journal of Molecular Endocrinology·Aristides LytrasPeter A Cattini
Jun 18, 2002·The Journal of Biological Chemistry·Blair B MadisonDeborah L Gumucio
Jul 12, 2003·Blood·Eleni Z KatsantoniJohn Strouboulis
Dec 14, 1999·DNA and Cell Biology·S S CaseJ A Lloyd
Jan 5, 2002·Journal of Virology·Dmitry M ShayakhmetovAndré Lieber
Aug 17, 2005·Journal of Virology·Hongjie WangAndré Lieber
Aug 4, 2001·Molecular and Cellular Biology·J Yu, J E Russell
Jul 21, 1998·Annals of the New York Academy of Sciences·Q LiG Stamatoyannopoulos
Jan 1, 1999·Hematology·J M McinerneyC H Lowrey
Feb 14, 1998·Gene·R HardisonW Miller
Aug 5, 1998·Current Opinion in Genetics & Development·R A Swank, G Stamatoyannopoulos