Shematrin: a family of glycine-rich structural proteins in the shell of the pearl oyster Pinctada fucata

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Masato YanoHiroshi Miyamoto

Abstract

Random sequencing of molecules from a cDNA library constructed from mantle mRNA of the pearl oyster Pinctada fucata was used to obtain information on organic matrix proteins in the shell. In the determined sequences, we identified 7 distinct cDNAs encoding similar glycine-rich domains. Complete sequence analysis of these cDNAs showed that the predicted sequences of the proteins, which we named shematrins, possessed similar domains comprising repeat sequences of two or more glycines, followed by a hydrophobic amino acid. In addition, in shematrin-1, -2 and -3, a repeat domain designated as XGnX (where X is a hydrophobic amino acid) was conserved. It is of further note that all the shematrin proteins have RKKKY, RRKKY or RRRKY as their C-terminal sequence. According to northern blot analysis, all shematrins are exclusively expressed in the mantle, and particularly in the edge region of the mantle; furthermore, peptide fragments similar to shematrin-1 and -2 were detected in the prismatic layer of shells by MALDI-TOF/TOF MS analysis. These findings suggest that many of shematrins are synthesized in the mantle edge and secreted into the prismatic layer of the shell, where the protein family is thought to provide a framework for cal...Continue Reading

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Citations

Feb 7, 2008·Marine Biotechnology·Daisuke TakakuraTetsuro Samata
Mar 15, 2012·The Journal of Biological Chemistry·Dong FangRongqing Zhang
Feb 9, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takeshi TakeuchiNori Satoh
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Sep 12, 2007·BMC Evolutionary Biology·Daniel J JacksonBernard M Degnan
Jul 9, 2014·Acta Biochimica Et Biophysica Sinica·Ya LinRongqing Zhang
Apr 28, 2012·Gene·Chihiro NogawaTetsuro Samata
Dec 27, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Céline PavatFrédéric Marin
Nov 26, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yoko MiyazakiTetsuro Samata
Sep 12, 2014·Biotechnology Journal·Yun Jung YangHyung Joon Cha
Dec 8, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kouhei NagaiHiroshi Miyamoto
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Apr 2, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ganchu JiaRongqing Zhang
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahesh Kumar Sah, Subha Narayan Rath

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