Shengmaisan regulates pacemaker potentials in interstitial cells of cajal in mice

Journal of Pharmacopuncture
Byung Joo Kim

Abstract

Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of diverse organs in Korea. The interstitial cells of Cajal (ICCs) are pacemaker cells that play an important role in the generation of coordinated gastrointestinal (GI) motility. We have aimed to investigate the effects of SMS in the ICCs in the mouse small intestine. To dissociate the ICCs, we used enzymatic digestions from the small intestine in a mouse. After that, the ICCs were identified immunologically by using the anti-c-kit antibody. In the ICCs, the electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICCs. The ICCs generated pacemaker potentials in the mouse small intestine. SMS produced membrane depolarization with concentration-dependent manners in the current clamp mode. Pretreatment with a Ca(2+) free solution and thapsigargin, a Ca(2+)-ATPase inhibitor in the endoplasmic reticulum, stopped the generation of the pacemaker potentials. In the case of Ca(2+)-free solutions, SMS induced membrane depolarizations. However, when thapsigargin in a bath solution was applied, the membrane depolarization was not produced by SMS. The membrane depolarizations produced by S...Continue Reading

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Citations

Jul 27, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jin-Yong JoungChang-Gue Son
Feb 13, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jin MaHuanlin Wu

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Methods Mentioned

BETA
dissection

Software Mentioned

pClamp
Origin

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Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

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