Abstract
Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of diverse organs in Korea. The interstitial cells of Cajal (ICCs) are pacemaker cells that play an important role in the generation of coordinated gastrointestinal (GI) motility. We have aimed to investigate the effects of SMS in the ICCs in the mouse small intestine. To dissociate the ICCs, we used enzymatic digestions from the small intestine in a mouse. After that, the ICCs were identified immunologically by using the anti-c-kit antibody. In the ICCs, the electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICCs. The ICCs generated pacemaker potentials in the mouse small intestine. SMS produced membrane depolarization with concentration-dependent manners in the current clamp mode. Pretreatment with a Ca(2+) free solution and thapsigargin, a Ca(2+)-ATPase inhibitor in the endoplasmic reticulum, stopped the generation of the pacemaker potentials. In the case of Ca(2+)-free solutions, SMS induced membrane depolarizations. However, when thapsigargin in a bath solution was applied, the membrane depolarization was not produced by SMS. The membrane depolarizations produced by S...Continue Reading
References
Jan 26, 1995·Nature·J D HuizingaA Bernstein
Oct 1, 1994·The Journal of Physiology·S M WardK M Sanders
Aug 1, 1996·Gastroenterology·K M Sanders
Oct 23, 1998·The Journal of Physiology·S D KohS M Ward
Dec 23, 1998·Journal of the Autonomic Nervous System·R HaggerD Kumar
Feb 6, 1999·Journal of the Autonomic Nervous System·S TorihashiY Watanabe
May 12, 2000·Journal of Clinical Pharmacology·J T Cheng
Jun 2, 2000·The Journal of Physiology·S M WardK M Sanders
Jul 5, 2003·Biological & Pharmaceutical Bulletin·Lei WangTetsuya Konishi
Nov 1, 2003·The American Journal of Chinese Medicine·Haruyo IchikawaTetsuya Konishi
May 10, 2005·Journal of Pharmacological Sciences·Nien-Lu WangMao-Tsun Lin
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kyung-Jong WonSean M Ward
Nov 16, 2005·Gastroenterology·Byung Joo KimKi Whan Kim
Mar 16, 2006·Journal of Pharmacological Sciences·Takashi SakamotoSeiichi Komori
Mar 23, 2006·The American Journal of Chinese Medicine·Jyh-Sheng YouYing-Shiung Lee
Aug 3, 2006·The American Journal of Chinese Medicine·Haruyo IchikawaTetsuya Konishi
May 9, 2007·Journal of Pharmacological Sciences·Hiroshi NishidaTetsuya Konishi
Mar 28, 2009·Molecules and Cells·Seok ChoiMirim Jin
Jan 21, 2011·Journal of Pharmaceutical and Biomedical Analysis·Na XuDanni Zhu
Aug 7, 2013·Pediatric Surgery International·Stefan Gfroerer, Udo Rolle
Citations
Nov 9, 2019·Current Molecular Medicine·Heng YangYuan-Hai Li
May 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chunhua LiuJin Qi
Jun 29, 2018·Pharmaceutical Biology·Ji Eun KimDae Youn Hwang
Sep 5, 2020·Food Science & Nutrition·Yuyuan LiBinbin Hou
Jan 27, 2017·BMC Gastroenterology·Ji Eun KimDae Youn Hwang
Dec 7, 2016·Molecular Medicine Reports·Ji Eun KimDae Youn Hwang
Jan 31, 2020·Trials·Yeon Hee BhangBeom-Joon Lee
Jul 27, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jin-Yong JoungChang-Gue Son
Feb 13, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jin MaHuanlin Wu