SHH expression in placental tissues and trophoblast cell oxidative stress injury during preeclampsia

European Review for Medical and Pharmacological Sciences
Q-P LiaoJ-Y Yan

Abstract

To assess the association between Sonic hedgehog (SHH) protein expression levels on placental tissues and the presence of preeclampsia and to assess oxidative stress in cultured trophoblast cells from placentas of women with preeclampsia treated under different conditions. We enrolled 30 pregnant women with early-onset preeclampsia (<34 weeks), 30 with late-onset preeclampsia (≥34 weeks), and 60 healthy pregnant women with early or late gestational ages for two control groups. We used ELISA tests to measure individual serum SHH levels. Also, we used real-time PCR, and Western blotting to detect Shh transcript and protein levels in placental tissues, respectively, and immunohistochemistry to localize the protein in placental tissues. We isolated trophoblast cells from placentas and cultured them in vitro under different conditions to compare oxidative stress injury and apoptosis rates between them. The mean serum SHH levels in early and late preeclampsia groups were similar (p>0.05). Placentas of women with and without preeclampsia both expressed SHH protein mainly in syncytiotrophoblast cytoplasms. The mean placental SHH protein expressions in both women with early-onset and late-onset preeclampsia were significantly higher tha...Continue Reading

Citations

Mar 28, 2021·Journal of Assisted Reproduction and Genetics·Yi HuangHui Li

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