Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study.

Nucleic Acids Research
Tomás SpringerJirí Homola

Abstract

Solid-phase hybridization, i.e. the process of recognition between DNA probes immobilized on a solid surface and complementary targets in a solution is a central process in DNA microarray and biosensor technologies. In this work, we investigate the simultaneous effect of monovalent and divalent cations on the hybridization of fully complementary or partly mismatched DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrate that the hybridization process is substantially influenced by the cation shielding effect and that this effect differs substantially for solid-phase hybridization, due to the high surface density of negatively charged probes, and hybridization in a solution. In our study divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium (15 mM Mg2+ in buffer led to significantly higher hybridization than even 1 M Na+). This trend is opposite to that established for oligonucleotides in a solution. It is also shown that solid-phase duplex destabilization substantially increases with the length of the involved oligonucleotides. Moreover, it is demonstrated that the use of a buffer with the appropriate cation composition c...Continue Reading

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Citations

Apr 9, 2013·Analytica Chimica Acta·Hana Šípová, Jiří Homola
Dec 28, 2012·Analytical Chemistry·Sourav MishraRupa Mukhopadhyay
Mar 4, 2011·The Analyst·David A C ThomsonMatthew A Cooper
Mar 31, 2016·Nucleic Acids Research·Sourav MishraRupa Mukhopadhyay
Feb 1, 2015·Analytical and Bioanalytical Chemistry·Markéta BockováJiří Homola
Sep 13, 2014·Biosensors & Bioelectronics·Karel KnezJeroen Lammertyn
Aug 12, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Sourav MishraRupa Mukhopadhyay
Feb 7, 2014·The Analyst·Blake N Johnson, Raj Mutharasan
Jul 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hui ChengRenjun Pei
Jan 26, 2021·Essays in Biochemistry·Rebecca Andrews
Jan 8, 2021·ACS Applied Materials & Interfaces·Nako NakatsukaAnne M Andrews
Feb 7, 2021·European Journal of Immunology·Julia Kennedy-DarlingGarry P Nolan
Apr 3, 2021·Biosensors & Bioelectronics·Meenu SelvarajFabio Biscarini
Aug 25, 2020·Analytical Chemistry·Sagun JonchheHanbin Mao

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Methods Mentioned

BETA
biosensor
surface plasmon resonance
chip
chips

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