PMID: 2110828Jan 23, 1990Paper

Shift of binding site at the interface between actin and myosin

Biochemistry
K Yamamoto

Abstract

The molar ratio dependent change in the binding manner between actin and the lysine-rich sequence at the junction between 50K and 20K domains of subfragment 1 was studied by both protease digestion and cross-linking with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide. The tryptic cleavage site at the function between 50K and 20K was found to be located between the third and fourth lysine residues in the lysine-rich sequence -KKGGKKK-. This site was not protected by actin when the molar ratio of actin to subfragment 1 was 1:1 but was protected at 2:1 and 3:1. The V8 protease cleavage site of chicken subfragment 1 and the elastase cleavage site of rabbit subfragment 1 were found to be located four residues away from the N-terminus of the lysine-rich sequence. Unlike the tryptic cleavage site, this site was protected by actin more when the molar ratio of actin to subfragment 1 was 1:1 than when it was 2:1 and 3:1. To understand the reason for the opposite effect of the molar ratio observed at the middle of and at four residues away from the lysine-rich sequence, actual cross-linked residue(s) was (were) determined by subjecting cross-linked product to a protein sequencer. It was found that the cross-linked sites were mainly at th...Continue Reading

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Citations

Dec 16, 1998·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Y SugawaraM Imamaki
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·A A BobkovE Reisler
Dec 15, 1992·European Journal of Biochemistry·E Blotnick, A Muhlrad
May 31, 1991·Biochemical and Biophysical Research Communications·O A Andreev, J Borejdo
Sep 15, 1993·Structure·M K Reedy
Nov 16, 1993·Biochemistry·O A AndreevJ Borejdo
Nov 14, 1997·Circulation Research·O A Andreev, J Borejdo
Oct 1, 1992·Journal of Muscle Research and Cell Motility·O A Andreev, J Borejdo
May 24, 1996·The Journal of Biological Chemistry·L BlanchoinD Pantaloni
Oct 24, 2000·The Journal of Biological Chemistry·P B JoelH L Sweeney

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