Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum ∼1 kPa) and U251 glioma cells (optimum ∼100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.
Possible future issues in the treatment of glioblastomas: special emphasis on cell migration and the resistance of migrating glioblastoma cells to apoptosis
Determinants of maximal force transmission in a motor-clutch model of cell traction in a compliant microenvironment
Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity
Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction
Modeling and predictions of biphasic mechanosensitive cell migration altered by cell-intrinsic properties and matrix confinement
The Effects of Stiffness, Fluid Viscosity, and Geometry of Microenvironment in Homeostasis, Aging, and Diseases: A Brief Review.
Application of External Force Regulates the Migration and Differentiation of Adipose-Derived Stem/Progenitor Cells by Altering Tissue Stiffness
A glance on the role of actin in osteogenic and adipogenic differentiation of mesenchymal stem cells
The Role of Stiffness in Cell Reprogramming: A Potential Role for Biomaterials in Inducing Tissue Regeneration
A novel method to make viscoelastic polyacrylamide gels for cell culture and traction force microscopy.
Recapitulating in vivo-like plasticity of glioma cell invasion along blood vessels and in astrocyte-rich stroma
The glycocalyx core protein Glypican 1 protects vessel wall endothelial cells from stiffness-mediated dysfunction and disease.
Synthesis of aligned porous polyethylene glycol/silk fibroin/hydroxyapatite scaffolds for osteoinduction in bone tissue engineering.
Cell Shape and Durotaxis Explained from Cell-Extracellular Matrix Forces and Focal Adhesion Dynamics
Human mammary epithelial cells in a mature, stratified epithelial layer flatten and stiffen compared to single and confluent cells.
Combinatorial mathematical modelling approaches to interrogate rear retraction dynamics in 3D cell migration.
Fibronectin Patches as Anchoring Points for Force Sensing and Transmission in Human Induced Pluripotent Stem Cell-Derived Pericytes.
Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair.
Fundamental Characteristics of Neuron Adhesion Revealed by Forced Peeling and Time-Dependent Healing.
Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing.
Adhesion Molecules in Health and Disease
Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.
Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.