Shifting the optimal stiffness for cell migration

Nature Communications
Benjamin L BangasserDavid J Odde

Abstract

Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum ∼1 kPa) and U251 glioma cells (optimum ∼100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.

References

Jul 1, 1991·Biophysical Journal·P A DiMillaD A Lauffenburger
Aug 1, 1989·The Journal of Cell Biology·S L GoodmanK von der Mark
Nov 1, 1988·Neuron·T Mitchison, M Kirschner
May 1, 1981·Analytical Biochemistry·J D Aplin, R C Hughes
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Feb 8, 2002·American Journal of Physiology. Cell Physiology·James P ButlerJeffrey J Fredberg
Apr 20, 2002·Biophysical Journal·Emilios K DimitriadisRichard S Chadwick
Feb 12, 1962·Biochimica Et Biophysica Acta·M KASAIF OOSAWA
Apr 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Florence LefrancRobert Kiss
May 23, 2008·Molecular Biology of the Cell·Christopher BeadlePeter Canoll
Dec 17, 2008·Science·Clarence E Chan, David J Odde
Jan 23, 2009·Nature Cell Biology·Kaylene J SimpsonJoan S Brugge
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amit Pathak, Sanjay Kumar
Jan 28, 2014·Cellular and Molecular Bioengineering·Benjamin L Bangasser, David J Odde
May 6, 2014·Nature Materials·Alberto Elosegui-ArtolaPere Roca-Cusachs
Feb 20, 2015·Nature Communications·Ovijit ChaudhuriDavid J Mooney

Citations

Dec 7, 2017·International Journal of Molecular Sciences·Kenta MasuiNoriyuki Shibata
Oct 27, 2017·Cell Biochemistry and Biophysics·Michael ReimerJoseph Martin Schober
Oct 17, 2018·Molecular Biology of the Cell·Devin B MairRong Li
Jul 24, 2019·Soft Matter·Ahmet Nihat SimsekBenedikt Sabass
Nov 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yizeng LiSean X Sun
Jan 11, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Nieves MovillaJose Manuel García-Aznar
Oct 8, 2019·PLoS Computational Biology·Avery ParrDaniel A Hammer
May 23, 2020·Human Reproduction Update·Yassen AbbasAshley Moffett
Aug 22, 2020·Tissue Engineering. Part B, Reviews·XiHang ChenYi Yuan
May 5, 2020·BioImpacts : BI·Maryam Sadat KhoramgahHossein Ghanbarian
Mar 14, 2019·Nature Communications·William Y WangBrendon M Baker
Aug 25, 2019·Essays in Biochemistry·James R W Conway, Guillaume Jacquemet
Jul 18, 2020·Stem Cell Research & Therapy·Asmat Ullah KhanJingxing Dai
Jun 12, 2019·Nature Reviews. Molecular Cell Biology·Jenny Z KechagiaPere Roca-Cusachs
Nov 22, 2018·Nature Communications·Erdem D TabdanovPaolo P Provenzano
Jun 27, 2019·Macromolecular Bioscience·Birgit StubbePeter Dubruel
Jun 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hannah S PicarielloSteven S Rosenfeld
Sep 11, 2019·Physical Review. E·Partho Sakha De, Rumi De
Aug 28, 2020·Nature·Ovijit ChaudhuriVivek B Shenoy
Aug 14, 2020·Biomechanics and Modeling in Mechanobiology·Elisabeth E CharrierPaul A Janmey
Aug 22, 2017·Histochemistry and Cell Biology·Pavlo GritsenkoPeter Friedl
Dec 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tristan P DriscollMartin A Schwartz
Nov 6, 2020·Biomaterials Science·Kamol DeyLuciana Sartore
Dec 19, 2020·Computational and Structural Biotechnology Journal·Michael Mak
Dec 9, 2020·Developmental Cell·Adam Shellard, Roberto Mayor
Nov 17, 2020·APL Bioengineering·Gabriel ShatkinAdam J Engler
Oct 24, 2018·Biophysical Journal·Zaw WinPatrick W Alford
Mar 9, 2021·Biomechanics and Modeling in Mechanobiology·Jonas F EichingerChristian J Cyron
Mar 11, 2021·PLoS Computational Biology·Joseph H R HetmanskiPatrick T Caswell
Jun 18, 2018·Biomaterials·A D SchwartzS R Peyton
Feb 20, 2021·The FEBS Journal·Nikos K KaramanosMaurizio Onisto
Apr 26, 2021·Journal of Experimental & Clinical Cancer Research : CR·Emilie MarhuendaNorbert Bakalara
Apr 21, 2021·Nature Materials·Kolade AdebowaleOvijit Chaudhuri
May 6, 2020·Current Biology : CB·Raimon Sunyer, Xavier Trepat

Datasets Mentioned

BETA
GSE95680

Methods Mentioned

BETA
transfection

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

El Protesista dental
A E Delamano
Molecular Biology of the Cell
Kathleen M Trybus, Vladimir I Gelfand
© 2021 Meta ULC. All rights reserved