Abstract
Oral diseases are biofilm-mediated diseases caused by imbalances in the ecology of resident microflora. Among them, dental caries (tooth decay) is considered the most common disease worldwide, and toothbrushing, which physically eliminates the oral biofilm, is the most widespread prevention strategy. Although it is well established that fluoride increases enamel resistance to acidic pH and promotes tooth remineralization, its effect on the biofilm bacterial communities' composition and metabolism is not fully understood. We have grown in vitro oral biofilms and used 16S rRNA Illumina sequencing to study the effect of fluoride on DNA- and RNA-based bacterial populations. In addition, a metatranscriptomic approach has also been performed, in which total RNA has been sequenced to study gene expression profiles in the presence/absence of 500 ppm sodium fluoride. Our data show a lower pH drop and a clear shift in total and metabolically active bacterial composition after fluoride exposure. Streptococcus oralis was the species most affected, with a 10-fold reduction in both DNA and RNA samples, whereas Rothia mucilaginosa underwent an 8-fold increase in the DNA and S. salivarius a 4- and 5-fold increase in the RNA and DNA samples, re...Continue Reading
References
Feb 1, 1990·Journal of Dental Research·I R Hamilton
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 1, 1987·Journal of Dental Research·F LagerlöfJ Ekstrand
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Nov 30, 2000·FEMS Microbiology Letters·R A Burne, R E Marquis
May 19, 2001·Caries Research·C Van Loveren
Aug 29, 2006·BMC Oral Health·Philip D Marsh
Aug 17, 2010·Bioinformatics·Robert C Edgar
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Sep 24, 2011·Journal of Dental Research·N Takahashi, J Washio
Jan 11, 2012·BMC Oral Health·Ella A NaumovaWolfgang H Arnold
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Dec 5, 2012·Caries Research·B NyvadD Beighton
Feb 23, 2013·British Dental Journal·J M ten Cate
Oct 2, 2013·Caries Research·A Simón-SoroA Mira
Apr 29, 2014·BMC Genomics·Alfonso Benítez-PáezAlex Mira
Jul 26, 2014·Bioinformatics·Donovan H ParksRobert G Beiko
Jan 31, 2015·Caries Research·Xuelian HuangMarcelle M Nascimento
May 18, 2016·FEMS Microbiology Letters·Takatoshi Murata, Nobuhiro Hanada
Jul 20, 2017·Scientific Reports·Ru LiEhsan Khafipour
Jul 28, 2017·Journal of Oral Microbiology·Ying LiaoDong Mei Deng
Dec 2, 2017·Journal of Dental Research·B T RosierA Mira
Jun 15, 2018·The ISME Journal·Majda DzidicAlex Mira
Aug 17, 2018·International Journal of Oral Science·Xin XuXuedong Zhou
Sep 12, 2018·High-throughput·Daniela BergerZvi Loewy
Aug 28, 2019·Journal of Dental Research·M M NascimentoR A Burne