Shifts in Composition and Activity of Oral Biofilms After Fluoride Exposure.

Microbial Ecology
A López-López, A Mira

Abstract

Oral diseases are biofilm-mediated diseases caused by imbalances in the ecology of resident microflora. Among them, dental caries (tooth decay) is considered the most common disease worldwide, and toothbrushing, which physically eliminates the oral biofilm, is the most widespread prevention strategy. Although it is well established that fluoride increases enamel resistance to acidic pH and promotes tooth remineralization, its effect on the biofilm bacterial communities' composition and metabolism is not fully understood. We have grown in vitro oral biofilms and used 16S rRNA Illumina sequencing to study the effect of fluoride on DNA- and RNA-based bacterial populations. In addition, a metatranscriptomic approach has also been performed, in which total RNA has been sequenced to study gene expression profiles in the presence/absence of 500 ppm sodium fluoride. Our data show a lower pH drop and a clear shift in total and metabolically active bacterial composition after fluoride exposure. Streptococcus oralis was the species most affected, with a 10-fold reduction in both DNA and RNA samples, whereas Rothia mucilaginosa underwent an 8-fold increase in the DNA and S. salivarius a 4- and 5-fold increase in the RNA and DNA samples, re...Continue Reading

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Citations

Jul 2, 2021·Frontiers in Cellular and Infection Microbiology·Bob T RosierAlex Mira

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Datasets Mentioned

BETA
ERS4145783-ERS4145810
ERS4145811-ERS4145822

Methods Mentioned

BETA
Nucleic Acid Extraction
Illumina sequencing
Assay
PCA

Software Mentioned

Dada2 pipeline
PRINSEQ
custom
STAMP
FLASH
TRANSEQ
bowtie2
HMMER
megaBLAST
MEGAHIT

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