Shifts in the Holstein dairy cow milk fat globule membrane proteome that occur during the first week of lactation are affected by parity

Journal of Animal Science and Biotechnology
Mallory C HonanS L Greenwood

Abstract

The milk fat globule membrane (MFGM) proteomes of colostrum and transition milk are rich sources of proteins that are likely important for neonatal calf health. In addition, characterization of these proteomes could also yield valuable information regarding mammary gland physiology of the early postpartum lactating cow. The objectives of this research were to characterize the MFGM proteomes of colostrum and transition milk through sample collections at four timepoints postpartum, including the first milking (M1, colostrum), second milking (M2, transition milk), fourth milking (M4, transition milk), and fourteenth milking (M14, mature milk), and compare these proteomes between multiparous (MP; n = 10) and primiparous (PP; n = 10) Holstein dairy cows. Isolated MFGM proteins were labeled using Tandem Mass tagging and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein identification was completed using MASCOT and Sequest in Proteome Discoverer 2.2. The scaled abundance values were analyzed using PROC MIXED in SAS to determine the effects of milking (MIL), parity (PAR), and MIL × PAR. The adaptive false-discovery rate (FDR)-adjusted P values were determined using PROC MULTTEST. Protein characterization...Continue Reading

References

Jul 1, 1982·Journal of Reproduction and Fertility·C H Knight, M Peaker
May 20, 2000·Small Ruminant Research : the Journal of the International Goat Association·A SeviG Annicchiarico
Jun 4, 2002·DNA and Cell Biology·Emanuela Tolosano, Fiorella Altruda
Jul 3, 2003·Journal of Dairy Science·C E OntsoukaJ W Blum
Jun 15, 2006·Journal of Dairy Science·K KimuraJ P Goff
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Horst RobenekNicholas J Severs
Jul 26, 2006·Journal of Animal Science·H C FreetlyT Brown-Brandl
Nov 16, 2006·Journal of Dairy Science·N MillerP Lacasse
Nov 12, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ann Louise Worsøe JørgensenJan Stagsted
Dec 1, 2009·Journal of Proteomics·Michael AffolterMartin Kussmann
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Oct 27, 2011·Annals of Clinical Biochemistry·Marek H Dominiczak, Muriel J Caslake
Jul 28, 2012·The Journal of Experimental Biology·Shelley L C LangW Don Bowen
Dec 19, 2012·Cell Calcium·Danna B ZimmerMichael F Criscitiello
Feb 22, 2014·Current Protein & Peptide Science·Lorenzo E Hernández-CastellanoAnastasio Argüello
Feb 3, 2015·PloS One·Domenica ScumaciDomenico Britti
Sep 27, 2015·Veterinary Research·Grant A SmolenskiThomas T Wheeler
Nov 3, 2015·Best Practice & Research. Clinical Endocrinology & Metabolism·Joris R DelangheMarijn M Speeckaert
Feb 6, 2017·Methods in Molecular Biology·Chuming ChenCathy H Wu
Aug 10, 2017·Nutrients·Hans DemmelmairBo Lönnerdal
Sep 28, 2017·Development·Emilia PeuhuJohanna Ivaska
Jun 9, 2018·Current Protein & Peptide Science·Bassam H SabhaElrashdy M Redwan
Jul 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oto HanušRobert Kala
Jan 8, 2019·Journal of Dairy Science·Sabrina L Greenwood, Mallory C Honan
Jun 19, 2019·The Journal of Dairy Research·Richard A ScuderiSabrina L Greenwood

❮ Previous
Next ❯

Software Mentioned

SAS
Sequest
PANTHER
Proteome Discoverer
BLAST
MASCOT

Related Concepts

Related Feeds

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.