Shifts in the Midgut/Pyloric Microbiota Composition within a Honey Bee Apiary throughout a Season

Microbes and Environments
Jane LudvigsenKnut Rudi

Abstract

Honey bees (Apis mellifera) are prominent crop pollinators and are, thus, important for effective food production. The honey bee gut microbiota is mainly host specific, with only a few species being shared with other insects. It currently remains unclear how environmental/dietary conditions affect the microbiota within a honey bee population over time. Therefore, the aim of the present study was to characterize the composition of the midgut/pyloric microbiota of a honey bee apiary throughout a season. The rationale for investigating the midgut/pyloric microbiota is its dynamic nature. Monthly sampling of a demographic homogenous population of bees was performed between May and October, with concordant recording of the honey bee diet. Mixed Sanger-and Illumina 16S rRNA gene sequencing in combination with a quantitative PCR analysis were used to determine the bacterial composition. A marked increase in α-diversity was detected between May and June. Furthermore, we found that four distinct phylotypes belonging to the Proteobacteria dominated the microbiota, and these displayed major shifts throughout the season. Gilliamella apicola dominated the composition early on, and Snodgrassella alvi began to dominate when the other bacteria...Continue Reading

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Citations

May 4, 2016·Nature Reviews. Microbiology·Waldan K Kwong, Nancy A Moran
Nov 23, 2017·Molecular Ecology·Kasie RaymannNancy A Moran
Jan 18, 2019·Insect Molecular Biology·S RomeroL J Foster
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Sep 21, 2021·Frontiers in Microbiology·Ana Cuesta-MatéMichael Poulsen

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Methods Mentioned

BETA
Illumina
PCR
dissection
dissecting
Illumina sequencing

Software Mentioned

ALS
BLAST
MCR
Quantitative Insights Into Microbial Ecology ( QIIME )
Matlab
Basic Local Alignment Search Tool ( BLAST )
LinReg PCR
CLC workbench
LinReg
uclust

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