Abstract
Inflammatory cytokines may enhance renal injury in post-diarrheal hemolytic uremic syndrome (Stx HUS) by enhancing the cytotoxic effect of Shiga toxins (Stx). The sources of inflammatory cytokines in Stx HUS are unclear. Since Stx-1 potently inhibits protein synthesis by glomerular epithelial cells (GEC) and increases cytokine release by renal epithelial cells, we examined Stx-1 regulation of cytokine production by human GEC. Stx-1 (and cycloheximide (CHX), another protein synthesis inhibitor) cytotoxicity, protein synthesis inhibition, and effect on interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor (TNF) release and mRNA levels were determined. Stx-1 alone had a modest stimulatory effect on inflammatory cytokine production by GEC that occurred at toxin concentrations ranging from minimal to 50% inhibition of protein synthesis. CHX, at concentrations that produced similar inhibition of protein synthesis, increased IL-1, IL-6, and TNF protein release and mRNA accumulation, but in a different time- and dose-dependent pattern than Stx. Lipopolysaccharide (LPS) did not change IL-1, but stimulated IL-6 and TNF production. LPS and Stx-1 combined stimulated production of all three cytokines to a greater extent than...Continue Reading
Citations
Jan 5, 2014·The Journal of Microbiology·Moo-Seung LeeVernon L Tesh
Apr 29, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Carla ZojaMarina Morigi
Mar 6, 2004·Microbial Pathogenesis·Patricia B EisenhauerDavid S Newburg
Aug 23, 2002·The New England Journal of Medicine·Joel L Moake
Feb 28, 2007·Hematology·Massimo Franchini
Dec 1, 2005·Kidney International·Mukut SharmaElias A Lianos
Jan 7, 2009·Infection and Immunity·Mitchell A PsotkaTom G Obrig
Dec 25, 2004·Infection and Immunity·J F PohlenzE A Dean-Nystrom
Sep 1, 2005·Blood·Leticia H NolascoJoel L Moake
Jan 16, 2009·Pediatrics International : Official Journal of the Japan Pediatric Society·Ichiro KamiokaUNKNOWN Japanese Society for Pediatric Nephrology
Dec 7, 2006·The American Journal of Pathology·Marina MorigiCarla Zoja
Mar 15, 2005·Cellular Signalling·W E ColpoysC M Thorpe
Aug 30, 2005·Thrombosis Research·Massimo FranchiniDino Veneri
Oct 15, 2014·Infection and Immunity·Teresa M DesRochersDavid L Kaplan
Oct 3, 2014·Endocrine·Federica BaruttaGabriella Gruden
Dec 29, 2011·The American Journal of Pathology·Maria V RamosMarina Palermo
Dec 8, 2017·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Carlamaria ZojaMarina Morigi
Mar 7, 2008·Journal of the American Society of Nephrology : JASN·Peter G Tipping
Nov 20, 2019·Pediatrics International : Official Journal of the Japan Pediatric Society·Masaki Shimizu
Nov 6, 2007·American Journal of Physiology. Renal Physiology·Volker Vallon
Sep 27, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maurizio BrigottiMarco A Cassatella
May 28, 2020·Toxins·Christian Menge
Oct 8, 2013·The Journal of Biological Chemistry·Nathan C LoJoel Moake
Oct 17, 2002·Renal Failure·Ji Eun LeeDong Kyu Jin
Jan 13, 2019·Microorganisms·Simona BuelliMarina Morigi
Jun 25, 2015·Microbiology Spectrum·Diana Karpman, Anne-Lie Ståhl