Shiga toxin binding to glycolipids and glycans.

PloS One
Karen M GallegosAlison A Weiss

Abstract

Immunologically distinct forms of Shiga toxin (Stx1 and Stx2) display different potencies and disease outcomes, likely due to differences in host cell binding. The glycolipid globotriaosylceramide (Gb3) has been reported to be the receptor for both toxins. While there is considerable data to suggest that Gb3 can bind Stx1, binding of Stx2 to Gb3 is variable. We used isothermal titration calorimetry (ITC) and enzyme-linked immunosorbent assay (ELISA) to examine binding of Stx1 and Stx2 to various glycans, glycosphingolipids, and glycosphingolipid mixtures in the presence or absence of membrane components, phosphatidylcholine, and cholesterol. We have also assessed the ability of glycolipids mixtures to neutralize Stx-mediated inhibition of protein synthesis in Vero kidney cells. By ITC, Stx1 bound both Pk (the trisaccharide on Gb3) and P (the tetrasaccharide on globotetraosylceramide, Gb4), while Stx2 did not bind to either glycan. Binding to neutral glycolipids individually and in combination was assessed by ELISA. Stx1 bound to glycolipids Gb3 and Gb4, and Gb3 mixed with other neural glycolipids, while Stx2 only bound to Gb3 mixtures. In the presence of phosphatidylcholine and cholesterol, both Stx1 and Stx2 bound well to Gb3 ...Continue Reading

References

Jan 1, 1975·Journal of Neuroscience Research·W T NortonG H DeVries
Aug 1, 1991·Molecular Microbiology·V L Tesh, A D O'Brien
Mar 1, 1991·Reviews of Infectious Diseases·A Donohue-RolfeG T Keusch
Apr 29, 1988·Biochemical and Biophysical Research Communications·T WaddellC Lingwood
Apr 1, 1996·Trends in Microbiology·C A Lingwood
Mar 4, 1998·Biochemistry·M D CummingsR J Read
Jun 9, 2000·Kidney International·A K HughesD E Kohan
Jan 26, 2002·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·M TakenagaY Mizushima
Aug 8, 2002·Kidney International·Niels W P RutjesClifford A Lingwood
Jan 3, 2007·Nature Cell Biology·Ken JacobsonRichard G W Anderson
May 29, 2007·Microbial Pathogenesis·Takeshi ShimizuTakashi Hamabata
Jan 10, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mark E ArnoldGeoff C Pritchard
Aug 16, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Johanna ScheiringLothar Bernd Zimmerhackl
Oct 3, 2008·Pediatrics in Review·Dilys A Whyte, Richard N Fine
Nov 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·H Jelger Risselada, Siewert J Marrink
Jan 31, 2009·Kidney International. Supplement·Martin Bitzan
May 28, 2009·Chembiochem : a European Journal of Chemical Biology·Daniel M LewallenSuri S Iyer
Aug 18, 2009·Expert Review of Anti-infective Therapy·Marina S PalermoGabriela C Fernández
Oct 27, 2009·Journal of Immunological Methods·Laurence HeinrichRichard Cohen

❮ Previous
Next ❯

Citations

Jul 2, 2014·PloS One·Sayali S Karve, Alison A Weiss
May 7, 2014·European Journal of Medicinal Chemistry·Xueer ZhaoMin Chen
Sep 4, 2013·Glycobiology·Robyn PetersonNicolle Packer
Jan 8, 2013·Molecular Immunology·Zohreh NademiChristopher Baldwin
Mar 21, 2015·Scientific Reports·Chelsea McCallisterNikolas Nikolaidis
Jun 1, 2015·Advanced Drug Delivery Reviews·Adam WeismanKenneth J Shea
Sep 11, 2012·Toxicon : Official Journal of the International Society on Toxinology·Jonas BerganKirsten Sandvig
Dec 3, 2014·Biochimica Et Biophysica Acta·Sahaja AigalWinfried Römer
Jan 10, 2017·Journal of Theoretical Biology·Dennis J YoonDaniel T Kamei
Dec 14, 2016·Carbohydrate Research·Dawa TseringHongzhi Cao
Jun 19, 2015·Clinical Microbiology Reviews·Laura Cooling
May 22, 2013·Infection and Immunity·Hailemichael O YosiefAlison A Weiss
Mar 24, 2016·Journal of Bacteriology·Christine A PellinoAlison A Weiss
Apr 16, 2016·PLoS Pathogens·Julie Elisabeth HeggelundUte Krengel
Mar 3, 2019·Glycobiology·Hyun-Kyu ChoiHung-Jen Wu
Jul 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David M P De OliveiraMartina L Sanderson-Smith
Oct 9, 2019·Scientific Reports·Karl E JohanssonDiana Karpman
Jun 12, 2020·Frontiers in Cellular and Infection Microbiology·Karl JohanssonDiana Karpman
Apr 11, 2018·Glycobiology·Jie ShiRoland J Pieters
Jan 19, 2021·The Journal of Biological Chemistry·Katarzyna Szymczak-KulusMarcin Czerwinski
Mar 15, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hsiang-Pu FengChia-Jung Yu
Jun 25, 2015·Microbiology Spectrum·Lothar Beutin, Patrick Fach
Dec 23, 2014·Microbiology Spectrum·Angela R Melton-Celsa
Jun 3, 2021·Toxins·Clifford Lingwood
Jun 12, 2020·Cell Host & Microbe·Erik Petersen, Samuel I Miller
Aug 5, 2021·Chemical Society Reviews·Yujun KimInjae Shin

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Surface Plasmon
PCR
protein folding
size exclusion chromatography
protein assay

Software Mentioned

GraphPad Prismâ¢
Prism
ORIGIN
Prism5
GraphPad

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Blood Coagulation Signaling Pathways

Coagulation is the process by which a blood clot is formed. This process includes both the formation of a platelet plug as well as a cascade of clotting factors resulting in the formation of fibrin strands. Find the latest research on coagulation signaling pathways here.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.