Shigella IpaB and IpaD displayed on L. lactis bacterium-like particles induce protective immunity in adult and infant mice

Immunology and Cell Biology
Shannon J HeineMarcela F Pasetti

Abstract

Shigella spp. are among the enteric pathogens with the highest attributable incidence of moderate-to-severe diarrhea in children under 5 years of age living in endemic areas. There are no vaccines available to prevent this disease. In this work, we investigated a new Shigella vaccine concept consisting of nonliving, self-adjuvanted, Lactococcus lactis bacterium-like particles (BLP) displaying Shigella invasion plasmid antigen (Ipa) B and IpaD and examined its immunogenicity and protective efficacy in adult and newborn/infant mice immunized via the nasal route. Unique advantages of this approach include the potential for broad protection due to the highly conserved structure of the Ipas and the safety and practicality of a probiotic-based mucosal/adjuvant delivery platform. Immunization of adult mice with BLP-IpaB and BLP-IpaD (BLP-IpaB/D) induced high levels of Ipa-specific serum IgG and stool IgA in a dose-dependent manner. Immune responses and protection were enhanced by BLP delivery. Vaccine-induced serum antibodies exhibited opsonophagocytic and cytotoxic neutralizing activity, and IpaB/D IgG titers correlated with increased survival post-challenge. Ipa-specific antibody secreting cells were detected in nasal tissue and lun...Continue Reading

References

Jul 9, 1992·Nature·A ZychlinskyP J Sansonetti
Jun 1, 1987·The Journal of Infectious Diseases·R E BlackS B Formal
Feb 1, 1994·Molecular Microbiology·A ZychlinskyP J Sansonetti
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·D S SouthamM D Inman
Jan 5, 2006·Applied and Environmental Microbiology·Tjibbe BosmaKees Leenhouts
Jan 18, 2006·Methods : a Companion to Methods in Enzymology·Maarten L van RoosmalenKees Leenhouts
Nov 4, 2006·Vaccine·Sandrine A L AudouyPeter W M Hermans
May 8, 2007·The Journal of Allergy and Clinical Immunology·Jennifer DebarryHolger Heine
Jan 9, 2010·Vaccine·Justen H PasswellUNKNOWN Israeli Shigella Study Group
Oct 16, 2010·Nature Reviews. Immunology·Martin F Bachmann, Gary T Jennings
Dec 29, 2011·Infection and Immunity·Francisco J Martinez-BecerraWendy L Picking
Jul 6, 2012·FEMS Immunology and Medical Microbiology·Soma MitraHemanta Koley
Mar 16, 2013·Protein Science : a Publication of the Protein Society·Shyamal P ChoudhariWendy L Picking
Apr 23, 2013·Vaccine·Francisco J Martinez-BecerraWendy L Picking
Jan 22, 2014·Nature Reviews. Immunology·Martin GuilliamsBart N Lambrecht
Jan 24, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shannon J HeineMarcela F Pasetti
Jun 12, 2014·Journal of Pharmaceutical Sciences·Shyamal P ChoudhariWendy L Picking
Jun 25, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Lillian L Van de Verg, Malabi M Venkatesan
Jun 25, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sofie LivioMyron M Levine

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Citations

Mar 1, 2016·Nature Reviews. Microbiology·Hao Chung TheStephen Baker
Apr 24, 2016·Pathogens and Disease·BreOnna C DeLaineEileen M Barry
Oct 21, 2016·Applied Microbiology and Biotechnology·Ruifeng MaoYefu Wang
Apr 6, 2017·Microbial Cell Factories·Adelene Ai-Lian SongRaha Abdul Rahim
Jan 19, 2019·Applied Microbiology and Biotechnology·Tina Vida Plavec, Aleš Berlec
Jul 10, 2019·Human Vaccines & Immunotherapeutics·Esther Ndungo, Marcela F Pasetti
Jun 10, 2015·Immunology and Cell Biology·Malabi M Venkatesan
Dec 12, 2018·PLoS Pathogens·Valeria CiancarellaMaria Lina Bernardini
Feb 23, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shannon J HeineMarcela F Pasetti
Sep 16, 2020·Archives of Microbiology·Ahtesham Ahmad Shad, Wajahat Ahmed Shad
May 18, 2021·Frontiers in Cellular and Infection Microbiology·Mariana L FerrariAnastasia D Gazi

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Methods Mentioned

BETA
ELISA
size exclusion chromatography
Electrophoresis
flow cytometry
lavages

Software Mentioned

Living Image
ZEN Imaging
GraphPad Prism
SAS
NCSS

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