Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization

Nature Microbiology
Jean-Yves TinevezBenoit Marteyn

Abstract

Pathogenic enterobacteria face various oxygen (O2) levels during intestinal colonization from the O2-deprived lumen to oxygenated tissues. Using Shigella flexneri as a model, we have previously demonstrated that epithelium invasion is promoted by O2 in a type III secretion system-dependent manner. However, subsequent pathogen adaptation to tissue oxygenation modulation remained unknown. Assessing single-cell distribution, together with tissue oxygenation, we demonstrate here that the colonic mucosa O2 is actively depleted by S. flexneri aerobic respiration-and not host neutrophils-during infection, leading to the formation of hypoxic foci of infection. This process is promoted by type III secretion system inactivation in infected tissues, favouring colonizers over explorers. We identify the molecular mechanisms supporting infectious hypoxia induction, and demonstrate here how enteropathogens optimize their colonization capacity in relation to their ability to manipulate tissue oxygenation during infection.

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Citations

Jul 22, 2020·Annual Review of Microbiology·Markus Kreuzer, Wolf-Dietrich Hardt
Sep 14, 2020·Immunology·Hannah Guak, Connie M Krawczyk
Jan 23, 2020·MBio·Carole J KuehlMatthew K Waldor
Jan 8, 2020·International Journal of Molecular Sciences·Louise InjarabianBenoit S Marteyn
Jul 2, 2020·Scientific Reports·Louise InjarabianBenoit S Marteyn
Oct 31, 2020·Cellular and Molecular Life Sciences : CMLS·Inaya HayekAnja Lührmann
Dec 10, 2020·Microorganisms·Waad BajunaidFrançois-Xavier Campbell-Valois
Apr 4, 2021·Immunology and Cell Biology·Louise InjarabianBenoit S Marteyn

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Software Mentioned

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