Shigella sonnei phase I and phase II: susceptibility to direct serum lysis and opsonic requirements necessary for stimulation of leukocyte redox metabolism and killing.

Infection and Immunity
G S Madonna, R C Allen

Abstract

The synthesis of the lipopolysaccharide O-specific repeat polymer by Shigella sonnei phase I is a clearly defined bacterial virulence factor necessary for penetrating epithelial cells; S. sonnei phase II does not synthesize this antigen and is uniformly avirulent. The serum opsonic requirements, relative to differences in gross lipopolysaccharide structure, were investigated by quantification and comparison of polymorphonuclear leukocyte (PMNL) metabolism and PMNL-mediated microbicidal action to phase I and phase II organisms, using normal and immune serum. The stimulation of PMNL O2-redox metabolism, as required for oxidative killing, was quantified by a chemiluminescent technique, using luminol as a chemilumigenic substrate. Susceptibility to direct serum or serum PMNL-mediated killing was evaluated by serum and serum-phagocytic killing assays. Stimulation of PMNL metabolism and phagocytic killing of S. sonnei phase I required opsonification by specific phase I antibody plus the classical pathway of complement. S. sonnei phase II was susceptible to direct complement-mediated serum killing. Likewise, opsonification of the phase II microbe, as measured by PMNL-associated chemiluminescence, was effected by complement in the abse...Continue Reading

References

Mar 8, 1976·Biochemical and Biophysical Research Communications·R C Allen, L D Loose
Mar 1, 1973·The Journal of Infectious Diseases·M M LevineJ P Libonati
Feb 1, 1973·Infection and Immunity·R L StjernholmJ A Harris
Feb 1, 1971·Applied Microbiology·A A Marucci, T C Fuller
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT

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Citations

Sep 15, 1984·Cancer·M L TroxellR C Allen
Mar 21, 2015·PLoS Pathogens·Mariaelena CaboniChristiane Gerke
Nov 1, 1996·Clinical and Diagnostic Laboratory Immunology·M M LiebermanC A Pinney
Nov 1, 1984·Infection and Immunity·F GaldieroF Tedesco
Jan 1, 1985·Infection and Immunity·J O'BrienS Leppla

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