SHIP2 controls plasma membrane PI(4,5)P2 thereby participating in the control of cell migration in 1321 N1 glioblastoma cells

Journal of Cell Science
William's Elong EdimoChristophe Erneux

Abstract

Phosphoinositides, particularly phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], are recognized by SHIP2 (also known as INPPL1) a member of the inositol polyphosphate 5-phosphatase family. SHIP2 dephosphorylates PI(3,4,5)P3 to form PI(3,4)P2; the latter interacts with specific target proteins (e.g. lamellipodin). Although the preferred SHIP2 substrate is PI(3,4,5)P3, PI(4,5)P2 can also be dephosphorylated by this enzyme to phosphatidylinositol 4-phosphate (PI4P). Through depletion of SHIP2 in the glioblastoma cell line 1321 N1, we show that SHIP2 inhibits cell migration. In different glioblastoma cell lines and primary cultures, SHIP2 staining at the plasma membrane partly overlaps with PI(4,5)P2 immunoreactivity. PI(4,5)P2 was upregulated in SHIP2-deficient N1 cells as compared to control cells; in contrast, PI4P was very much decreased in SHIP2-deficient cells. Therefore, SHIP2 controls both PI(3,4,5)P3 and PI(4,5)P2 levels in intact cells. In 1321 N1 cells, the PI(4,5)P2-binding protein myosin-1c was identified as a new interactor of SHIP2. Regulation of PI(4,5)P2 and PI4P content by SHIP2 controls 1321 N1 cell migration through the organization of focal adhesion...Continue Reading

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Citations

Jun 2, 2016·Biochemical and Biophysical Research Communications·William's Elong EdimoChristophe Erneux
Oct 16, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zydrune Polianskyte-PrauseSanna Lehtonen
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Feb 9, 2021·Frontiers in Molecular Neuroscience·Kunie AndoKarelle Leroy
Dec 6, 2020·International Journal of Molecular Sciences·Mariah P CsolleChristina A Mitchell
Mar 7, 2021·Cancers·Chiara PediconeWilliam G Kerr
Mar 16, 2021·Frontiers in Neuroscience·Kunie AndoKarelle Leroy

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