PMID: 3002667Nov 1, 1985Paper

Short- and long-term evaluation of normal and abnormal human thyroid cells in monolayer culture

Clinical Endocrinology
Terry F DaviesE W Friedman

Abstract

We have investigated the TSH responsiveness of normal and abnormal human thyroid cells cultured in the short term with high serum concentrations and for up to 6 months in a low serum, chemically-defined, medium. Cells from normal human thyroid tissue (n = 9), multinodular goitre (n = 6), benign follicular adenomata (n = 6), and differentiated thyroid carcinoma (n = 3) formed confluent monolayers which were sensitive to bovine TSH (bTSH) in concentrations greater than 25 microU/ml when assessed by the intracellular response of cyclic AMP at 7 d of culture. Such sensitivity was less than that observed with a continuously proliferating thyroid cell line (FRTL-5) derived from Fisher rat thyroid and which responded to concentrations of bTSH as low as 5-10 microU/ml. Human cells derived from iodine/antithyroid drug treated Graves' thyroid tissue (n = 6) were less sensitive than normal cells requiring up to 500 microU/ml bTSH to increase intracellular cyclic AMP and poorly differentiated thyroid cancer cells (n = 3) failed to respond to bTSH. Long-term human thyroid cultures of normal and follicular adenoma cells in the chemically-defined medium used for the FRTL-5 cells had absent fibroblast growth and continued in monolayer form wit...Continue Reading

References

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Citations

May 1, 1987·Molecular and Cellular Endocrinology·D W WilliamsE D Williams
Dec 1, 1988·Molecular and Cellular Endocrinology·A MartinTerry F Davies
Dec 1, 1991·Molecular and Cellular Endocrinology·S R PageS S Nussey
Feb 5, 1996·Molecular and Cellular Endocrinology·A K RasmussenU Feldt-Rasmussen
Jan 1, 1993·Thyroid : Official Journal of the American Thyroid Association·Y TomerTerry F Davies
Feb 1, 1995·Thyroid : Official Journal of the American Thyroid Association·T HoeltingO H Clark
Jan 1, 1988·Autoimmunity·S M DowerTerry F Davies
Dec 1, 1987·Clinical Endocrinology·A FeldmanTerry F Davies
Dec 16, 1987·Biochemical and Biophysical Research Communications·P P Roger, J E Dumont
Mar 1, 2000·Journal of Child Neurology·P NopoulosJ L Rapoport

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