Short bouts of mechanical loading are as effective as dexamethasone at inducing matrix production by human bone marrow mesenchymal stem cell

European Cells & Materials
A SittichokechaiwutGwendolen C Reilly

Abstract

Dexamethasone (Dex) is used widely to induce differentiation in human mesenchymal stem cells (hMSCs); however, using a pharmaceutical agent to stimulate hMSC differentiation is not the best choice for engineered tissue transplantation due to potential side-effects. The goal of the present study was to investigate the effects of dynamic compressive loading on differentiation and mineralized matrix production of hMSCs in 3D polyurethane scaffolds, using a loading regimen previously shown to stimulate mineralised matrix production of mature bone cells (MLO-A5). hMSCs were seeded in polyurethane scaffolds and cultured in standard culture media with or without Dex. Cell-seeded scaffolds were compressed at 5% global strain for 2 h on day 9 and then every 5 days in a media-filled sterile chamber. Samples were tested for mRNA expression of alkaline phosphatase (ALP), osteopontin (OPN), collagen type 1 (col 1) and runt-related transcription factor-2 (RUNX-212 h) after the first loading, cell viability by MTS assay and alkaline phosphatase activity at day 12 of culture and cell viability, collagen content by Sirius red and calcium content by alizarin red at day 24 of culture. Neither Dex nor loading had significant effects on cell viabil...Continue Reading

Citations

Jul 14, 2011·Regenerative Medicine·Yongsung HwangShyni Varghese
Sep 4, 2015·BoneKEy Reports·Robin Michael Delaine-SmithRobin Mark Howard Rumney
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Sasima PuwanunGwendolen C Reilly
Jan 26, 2018·International Journal of Molecular Sciences·Lifang HuAirong Qian
Sep 28, 2010·Regenerative Medicine·Gregory YourekGwendolen C Reilly
Nov 11, 2010·Human Gene Therapy·Yeong-Hoon ChoiChristof Stamm
Aug 1, 2015·Biomechanics and Modeling in Mechanobiology·Feihu ZhaoLaoise M McNamara
Jul 31, 2019·Journal of Tissue Engineering and Regenerative Medicine·Sophie SchreivogelAnsgar Petersen
Nov 28, 2012·Advanced Healthcare Materials·Richard A ThibaultF Kurtis Kasper
Jan 5, 2011·Cell Proliferation·C E SarrafM Eastwood
Jun 13, 2014·Journal of Tissue Engineering and Regenerative Medicine·Shunro MiyashitaMisako Nakashima
Mar 5, 2016·International Journal of Molecular Medicine·Dirk HenrichCaroline Seebach
Dec 14, 2017·Applied Biochemistry and Biotechnology·Birru BhaskarGwendolen C Reilly
Sep 19, 2020·Journal of Tissue Engineering·Adam J JanvierJames R Henstock
Apr 30, 2013·Journal of Tissue Engineering and Regenerative Medicine·Jolanda Rita VetschSandra Hofmann
Dec 24, 2015·Biofabrication·Jessica SnyderWei Sun
Jan 12, 2019·Tissue Engineering. Part B, Reviews·Nicole HauptmannKlaus Liefeith
Oct 8, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robin M Delaine-SmithGwendolen C Reilly
Jul 12, 2019·World Journal of Stem Cells·Diane R WagnerJiliang Li

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