Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria

Journal of Agricultural and Food Chemistry
Chao ZhongBernd Nidetzky

Abstract

Short-chain cello-oligosaccharides (COS; degree of polymerization, DP ≤ 6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show the intensification and scale up (20 mL; gram scale) of COS production to 93 g/L product and in 82 mol % yield from sucrose (0.5 M). The COS were comprised of DP 3 (33 wt %), DP 4 (34 wt %), DP 5 (24 wt %), and DP 6 (9 wt %) and involved minimal loss (≤10 mol %) to insoluble fractions. After isolation (≥95% purity; ≥90% yield), the COS were examined for growth promotion of probiotic strains. Benchmarked against inulin, trans-galacto-oligosaccharides, and cellobiose, COS showed up to 4.1-fold stimulation of cell density for Clostridium butyricum, Lactococcus lactis subsp. lactis, Lactobacillus paracasei subsp. paracasei, and Lactobacillus rhamnosus but were less efficient with Bifidobacterium sp. This study shows the COS as selectively functional carbohydrates with prebiotic potential and demonstrates their efficient enzymatic production.

References

Jul 1, 1992·Journal of Biochemistry·M KitaokaH Taniguchi
Jun 1, 1995·The Journal of Nutrition·G R Gibson, M B Roberfroid
Feb 24, 2001·The Journal of the Royal Society for the Promotion of Health·A D BlackwoodM F Chaplin
Apr 23, 2003·Carbohydrate Research·Seung-Heon YoonJohn F Robyt
Nov 5, 2003·Analytical Biochemistry·Yi-Heng Percival Zhang, Lee R Lynd
Feb 6, 2004·Biochemistry·Desiree SprogøeLars K Skov
May 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yi-Heng Percival Zhang, Lee R Lynd
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rodolphe BarrangouTodd R Klaenhammer
Jul 26, 2006·Critical Reviews in Food Science and Nutrition·Katrien SwennenJan A Delcour
Oct 7, 2006·Biotechnology Progress·Mickaël Desvaux
Dec 21, 2006·Journal of Agricultural and Food Chemistry·María Luz SanzRobert A Rastall
Feb 22, 2007·The Journal of Nutrition·Marcel Roberfroid
Jun 15, 2007·Applied and Environmental Microbiology·Mukund AdsulDigambar Gokhale
Nov 28, 2007·Applied and Environmental Microbiology·Roland J SiezenJohan E T van Hylckama Vlieg
Oct 28, 2008·Journal of Bacteriology·Yakir NatafYuval Shoham
Dec 1, 2004·Nutrition Research Reviews·Glenn R GibsonMarcel B Roberfroid
Apr 21, 2009·Journal of Bacteriology·Rodolphe BarrangouRobert F Roberts
Dec 22, 2009·Current Opinion in Chemical Biology·Jun-ichi Kadokawa, Shiro Kobayashi
Jan 11, 2011·Applied and Environmental Microbiology·Karina PokusaevaDouwe van Sinderen
May 11, 2012·Gut Microbes·Harry J FlintEvelyne Forano
Oct 12, 2012·Frontiers in Microbiology·Michael G Gänzle, Rainer Follador
Feb 14, 2013·Current Opinion in Chemical Biology·Hiroyuki NakaiKen'ichi Ohtsubo
Apr 24, 2013·Nutrients·Joanne Slavin
May 15, 2013·BMC Genomics·Joakim M AndersenTodd R Klaenhammer
Jan 1, 2014·Frontiers in Immunology·Angélica T VieiraFlaviano S Martins
May 27, 2014·International Journal of Environmental Research and Public Health·Sabina Fijan
Dec 24, 2014·Annual Review of Food Science and Technology·Yong Jun Goh, Todd R Klaenhammer
Oct 24, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Nadim CassirBernard La Scola
Jun 19, 2016·Current Opinion in Biotechnology·Jochen Wachtmeister, Dörte Rother
Nov 24, 2016·Environmental Microbiology·Mathias KleinElke Nevoigt
May 13, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hajime KatanoHisashi Kimoto
Jul 1, 2017·Journal of Agricultural and Food Chemistry·Koen BeerensTom Desmet
Sep 16, 2017·Nutrients·Paulina Markowiak, Katarzyna Śliżewska
Dec 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Suwapat KittibunchakulThu-Ha Nguyen

❮ Previous
Next ❯

Citations

Mar 30, 2021·World Journal of Microbiology & Biotechnology·Patrícia F ÁvilaRosana Goldbeck
May 11, 2021·Applied Microbiology and Biotechnology·Zorica UbiparipTom Desmet
Aug 16, 2021·Carbohydrate Research·Gregory S BulmerJolanda M van Munster

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF543301.1
AAQ20920.1
CDZ24361.1

Methods Mentioned

BETA
nuclear magnetic resonance
X-ray

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.