Short-chain fatty acid delivery: assessing exogenous administration of the microbiome metabolite acetate in mice

Physiological Reports
Tyler B ShubitowskiJennifer L Pluznick

Abstract

Short-chain fatty acids (SCFAs) are fermentation by-products of gut microbes which have been linked to positive effects on host physiology; the most abundant SCFA is acetate. Exogenous administration of acetate alters host metabolism, immune function, and blood pressure, making it a biologic of interest. The effects of acetate have been attributed to activation of G-protein-coupled receptors and other proteins (i.e., HDACs), often occurring at locations distant from the gut such as the pancreas or the kidney. However, due to technical difficulties and costs, studies have often delivered exogenous acetate without determining if systemic plasma acetate levels are altered. Thus, it is unclear to what extent each method of acetate delivery may alter systemic plasma acetate levels. In this study, we aimed to determine if acetate is elevated after exogenous administration by drinking water (DW), oral gavage (OG), or intraperitoneal (IP) injection, and if so, over what timecourse, to best inform future studies. Using a commercially available kit, we demonstrated that sodium acetate delivered over 21 days in DW does not elicit a measurable change in systemic acetate over baseline. However, when acetate is delivered by OG or IP injectio...Continue Reading

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Citations

Aug 21, 2019·Nutrients·Manuel A González HernándezEllen E Blaak
Jun 4, 2020·Physiology·Brian G PollJennifer L Pluznick
Jan 27, 2021·Trends in Pharmacological Sciences·Graeme MilliganAndrew B Tobin
Jan 9, 2021·The Journal of Pharmacology and Experimental Therapeutics·Brian G PollJennifer L Pluznick
Sep 23, 2021·Physiological Reports·Brian G PollJennifer L Pluznick

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BETA
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