Dec 1, 1975

Short-chain fatty acid synthesis in brain. Subcellular localization and changes during development

The Biochemical Journal
G L ReijnierseC J Van der Ber


Acetyl-CoA synthase (EC, Propionyl-CoA synthase (EC 6.2.1.-) and butyryl-CoA synthase (EC were measured in subcellular fractions prepared by primary and density-gradient fractionation from adult rat brain by a method resulting in recoveries close to 100%. Most of the activity of the three enzymes was recovered in the crude mitochondrial fraction. On subfractionation of this crude mitochondrial fraction with continuous sucrose density gradients, most of the activity of the three enzymes was found at a higher density than NAD+-isocitrate dehydrogenase and at about the same density as glutamate dehydrogenase, confirming earlier reported data for acetyl-CoA synthase. The finding that propionyl-CoA synthase and butyryl-CoA synthase had about the same distribution in the gradients as acetyl-CoA synthase adds support to the hypothesis that mitochondria involved in the metabolism of these short-chain fatty acids (all three of which have been shown to result in a rapid and high labelling of glutamine in vivo) form a distinct subpopulation of the total mitochondrial population. The three synthase activities were found to differ from each other in their rate of change and their subcellular localization during rat brain d...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
Establishment and Maintenance of Localization
Centrifugation, Density Gradient
Fatty Acids, Volatile
Enzymes, antithrombotic
Coenzyme A Ligases
Gene Products, Protein

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