Short-chain fatty acids and trichostatin A alter tight junction permeability in human umbilical vein endothelial cells

Nutrition
Makoto MiyoshiAtsushi Ohata

Abstract

The short-chain fatty acids (SCFAs), butyrate, propionate, and acetate, produced by bacterial fermentation of dietary fiber, can modulate the transcription of certain genes by inhibiting histone deacetylase in colonocytes and several other cell types in vitro. We previously reported that butyrate decreases tight junction permeability by activating lipoxygenase (LOX) in intestinal monolayer cells. In the present study, we evaluated the effects of SCFAs on tight junction permeability in an endothelial cell culture and their possible mechanisms of action via histone deacetylase inhibitor activity. We also investigated the factors modulating tight junction permeability. The effects of butyrate, propionate, and acetate on tight junction permeability in human umbilical vein endothelial cells were examined using Transwell chamber cultures. The contributions of inducible nitric oxide synthase (NOS), endothelial NOS, estrogen receptor, cyclo-oxygenase, and LOX to SCFAs' effects were also evaluated. The effects of SCFAs were compared with those of trichostatin A, a typical histone deacetylase inhibitor. Low concentrations of butyrate and propionate decreased paracellular permeability without inducing cell damage. However, acetate decreas...Continue Reading

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Citations

Nov 6, 2010·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Makoto MiyoshiYu Usami
Nov 12, 2015·World Journal of Gastroenterology : WJG·Makoto UsamiHayato Yamashita
May 30, 2017·Journal of Dairy Science·Svenja MeissnerJörg R Aschenbach
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Xiaona ChenHong Li
Jul 22, 2021·The Journal of Experimental Medicine·Zuojia ChenVijay K Kuchroo
Aug 28, 2021·International Journal of Molecular Sciences·Akane WadaMotonobu Nakamura

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