Short-chain fatty acids inhibit intestinal trefoil factor gene expression in colon cancer cells

The American Journal of Physiology
C P TranA Giraud

Abstract

Intestinal trefoil factor (ITF) gene expression was detected in five colon cancer cell lines. ITF was synthesized by mucous cells of LIM 1215 and LIM 1863 lines, from which it is secreted constitutively. The ITF mRNA transcript was estimated to be 0.6 kb. In LIM 1215 cells, the expression of ITF was potently and dose-dependently inhibited by short-chain fatty acids (butyrate > propionate > acetate) within 8 h of application. The inhibitory effect of butyrate was ablated by actinomycin D and preceded its effects on differentiation of LIM 1215 cells as indicated by induction of alkaline phosphatase activity and counting of periodic acid-Schiff-positive cells. The human ITF promoter contained an 11-residue consensus sequence with high homology to the butyrate response element of the cyclin D1 gene. Mobility shift assays show specific binding of this response element to nuclear protein extracts of LIM 1215 cells. We conclude that butyrate inhibits ITF expression in colon cancer cells and that this effect may be mediated transcriptionally and independently of its effects on differentiation.

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Citations

Apr 1, 2000·Applied and Environmental Microbiology·A BarcenillaH J Flint
Oct 3, 2013·PloS One·Yuan XueYongsheng Chang
Jul 23, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stine O RasmussenPer T Sangild
Oct 22, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Concepcion Fernandez-EstivarizThomas R Ziegler
Dec 17, 2002·The Journal of General and Applied Microbiology·Narito AsanumaTsuneo Hino
Nov 19, 2013·Critical Reviews in Food Science and Nutrition·S Padma Ishwarya, P Prabhasankar
Jul 4, 2003·The Journal of Nutrition·James R Davie
Oct 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·S L PenderT T MacDonald
Mar 8, 2002·The Journal of Physiology·Mark A CuffSoraya P Shirazi-Beechey

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