Oct 3, 2013

Short chain N-acyl homoserine lactone production by soil isolate Burkholderia sp. strain A9

Sensors
Jian-Woon ChenKok-Gan Chan

Abstract

In the bacteria kingdom, quorum sensing (QS) is a cell-to-cell communication that relies on the production of and response to specific signaling molecules. In proteobacteria, N-acylhomoserine lactones (AHLs) are the well-studied signaling molecules. The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil. Strain A9 was identified as Burkholderia sp. using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. AHL production by A9 was detected with two biosensors, namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Thin layer chromatography results showed N-hexanoylhomoserine lactone (C6-HSL) and N-octanoylhomoserine lactone (C8-HSL) production. Unequivocal identification of C6-HSL and C8-HSL was achieved by high resolution triple quadrupole liquid chromatography-mass spectrometry analysis. We have demonstrated that Burkholderia sp. strain A9 produces AHLs that are known to be produced by other Burkholderia spp. with CepI/CepR homologs.

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References

  • References25
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Citations

Mentioned in this Paper

Biosensors
Homoserine lactone, (S)-isomer
Anterior Horizontal Limb of Lateral Sulcus (Human Only)
DNA, Ribosomal
Chromatography, Qualitative; Thin Layer, Analyte Not Elsewhere Specified
Cell Communication
Lactones
Mass Spectrometry
Biosensing Techniques
Burkholderia

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