Short Communication An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues

Genetics and Molecular Research : GMR
R R OliveiraM G A Vincentz

Abstract

Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or epigenetic mark polymorphism is often desirable; for this, a robust protocol to isolate high-quality RNA and DNA simultaneously from the same sample is required. Although commercial kits and protocols are available, they are mainly optimized for animal tissues and, in general, restricted to RNA or DNA extraction, not both. In the present study, we describe an efficient and accessible method to extract both RNA and DNA simultaneously from the same sample of various plant tissues, using small amounts of starting material. The protocol was efficient in the extraction of high-quality nucleic acids from several Arabidopsis thaliana tissues (e.g., leaf, inflorescence stem, flower, fruit, cotyledon, seedlings, root, and embryo) and from other tissues of non-model plants, such as Avicennia schaueriana (Acanthaceae), Theobroma cacao (Malvaceae), Paspalum notatum (Poaceae), and Sorghum ...Continue Reading

Citations

Jan 24, 2018·Scientific Reports·Maria Augusta Crivelente HortaAnete Pereira de Souza
Dec 14, 2019·Molecular Ecology·Mariana Vargas CruzAnete Pereira de Souza
Apr 8, 2020·Molecular Biology and Evolution·Régis L CorrêaSantiago F Elena
May 17, 2021·Journal of Biotechnology·Déborah Aires AlmeidaAnete Pereira de Souza
May 31, 2021·The New Phytologist·Américo J C VianaMichel Vincentz

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