Short communication: identification of a novel HIV type 1 subtype H/J recombinant in Canada with discordant HIV viral load (RNA) values in three different commercial assays

AIDS Research and Human Retroviruses
John E KimM John Gill

Abstract

The presence of HIV-1 non-B subtypes is increasing worldwide. This poses challenges to commercial diagnostic and viral load (RNA) monitoring tests that are predominantly based on HIV-1 subtype B strains. Based on phylogenetic analysis of the gag, pol, and env gene regions, we describe the first HIV-1 H/J recombinant in Canada that presented divergent viral load values. DNA sequence analysis of the gag gene region further revealed that genetic diversity between this H/J recombinant and the primers and probes used in the bio-Merieux Nuclisens HIV-1 QT (Nuclisens) and Roche Amplicor Monitor HIV-1, v1.5 (Monitor) viral RNA assays can erroneously lead to undetectable viral load values. This observation appears to be more problematic in the Nuclisens assay. In light of increasing genetic diversity in HIV worldwide we recommend that DNA sequencing of HIV, especially in the gag gene region targeted by primers and probes used in molecular diagnostic and viral load tests, be incorporated into clinical monitoring practices.

References

Dec 11, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Alessandra AmendolaMaria R Capobianchi

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Citations

Feb 8, 2011·AIDS·Joris HemelaarUNKNOWN WHO-UNAIDS Network for HIV Isolation and Characterisation
Nov 26, 2010·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Tyler O'BryanM John Gill
Nov 13, 2010·Antiviral Research·Gonzalo YebraUNKNOWN Cohort of Spanish AIDS Research Network (CoRIS)
Nov 27, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Burkhard GreveWolfgang Göhde
Jul 20, 2011·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·LeeAnne M LuftDeirdre L Church
Jan 23, 2014·Journal of Immunological Methods·Ana M SanchezThomas N Denny
Aug 12, 2016·Journal of Clinical Microbiology·Mark M ManakLinda L Jagodzinski

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