Short communication: Occurrence and differentiation of Mycobacterium avium ssp. paratuberculosis (MAP) strains from milk of cows from herd with low prevalence of MAP

Journal of Dairy Science
J SzteynK Liedke

Abstract

Mycobacterium avium ssp. paratuberculosis (MAP) is an important pathogen responsible for the chronic progressive granulomatous enteritis known as paratuberculosis. None of the detection methods of MAP infection based on isolation of the bacterium is 100% sensitive or specific. In this article, we describe the comparison of 2 MAP detection methods: direct isolation of genetic material and culture, in individual and pooled milk samples. The genetic types of MAP detected in the samples were also identified. The study was performed in a herd of 321 cows; apparent herd seroprevalence was 3.43%. Seven of 11 individual milk samples from seropositive cows were positive by culture (and confirmed by PCR), whereas all 11 were positive by direct PCR. Of the 62 milk pools from seronegative animals, 15 were positive by culture (and confirmed by PCR) and 13 were positive by direct PCR. Using multiplex PCR and PCR-restriction enzyme analysis (PCR-REA) methods, C (cattle) and S (sheep)-types of mycobacteria were identified. Most of the genetic material tested belonged to C-type. Detection of the MAP type occurring in an infected herd can help track the source of infection. We suggest using genetic material isolated directly from pooled milk sam...Continue Reading

References

May 15, 2002·FEMS Microbiology Letters·Stina EnglundKarl Erik Johansson
Aug 24, 2007·The Lancet Infectious Diseases·Martin FellerMatthias Egger
Sep 27, 2008·Preventive Veterinary Medicine·Søren Saxmose Nielsen, Nils Toft
Jul 7, 2009·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·H ShankarR J Greenstein
Nov 19, 2010·Journal of Clinical Microbiology·Michele GastaldelliNicola Pozzato
Oct 26, 2011·The Veterinary Clinics of North America. Food Animal Practice·Michael T Collins
Mar 20, 2015·Frontiers in Immunology·Leonardo A Sechi, Coad Thomas Dow
Dec 25, 2017·The Veterinary Clinics of North America. Food Animal Practice·Marie-Eve Fecteau

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