Short duration waveforms recorded extracellularly from freely moving rats are representative of axonal activity

Frontiers in Neural Circuits
Ashlee A RobbinsJeremy M Barry

Abstract

While extracellular somatic action potentials from freely moving rats have been well characterized, axonal activity has not. We report direct extracellular tetrode recordings of putative axons whose principal feature is a short duration waveform (SDW) with an average peak-trough length less than 179 μs. While SDW recordings using tetrodes have previously been treated as questionable or classified as cells, we hypothesize that they are representative of axonal activity. These waveforms have significantly shorter duration than somatic action potentials, are triphasic and are therefore similar to classic descriptions of microelectrode recordings in white matter and of in vitro action potential propagation along axons. We describe SDWs recorded from pure white-matter tracts including the alveus and corpus callosum. Recordings of several SDWs in the alveus exhibit grid-like firing patterns suggesting these axons carry spatial information from entorhinal cortical neurons. Finally, we locally injected the GABAA agonist Muscimol into layer CA1 of the hippocampus while simultaneously recording somatic activity and SDWs on the same tetrodes. The persistent activity of SDWs during Muscimol inactivation of somatic action potentials indicat...Continue Reading

Citations

May 6, 2015·Experimental Neurology·Jeremy M BarryGregory L Holmes
Dec 23, 2014·Nature Neuroscience·Dion KhodagholyGyörgy Buzsáki
Nov 1, 2016·Nature Neuroscience·Peter LakatosMonica Noelle O'Connell
Sep 17, 2019·The Journal of Physiology·Ágnes KandrácsLucia Wittner
Jan 19, 2020·Journal of Computational Neuroscience·Harry TranValérie Louis-Dorr
Apr 17, 2020·Nature Communications·Dora E AngelakiJean Laurens
Mar 29, 2020·Science Advances·Abdulmalik ObaidNicholas A Melosh
Dec 2, 2020·Nature Communications·Shan JiangXiaoting Jia
Mar 10, 2021·The Journal of Physiology·Péter Barthó
Mar 23, 2021·Cerebral Cortex·Angelique C PaulkSydney S Cash
Jul 17, 2021·Scientific Data·Csaba HorváthRichárd Fiáth

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