Short exposure to tranexamic acid does not affect, in vitro, the viability of human chondrocytes

European Journal of Medical Research
Remo GoderecciVittorio Calvisi

Abstract

Only few studies have investigated the effect of topical application of tranexamic acid (TXA) on "minimally" invasive joint surgical procedures in which articular cartilage is preserved; for this reason, actually many surgeons avoid the use of topical TXA even if the disadvantage related to a blood loss can occur. The aim of this study was to evaluate the cytotoxicity, on human chondrocytes, of TXA at different concentrations and times of exposure and the mechanisms of cell death. Experiments were carried out on isolated human chondrocytes harvested from eight patients who underwent total knee replacement. Cell viability was determined using XTT assay and was assessed at 0, 24 and 48 h intervals after a 10-min-long treatment, followed by thorough washes, or at 24 and 48 h of treatment at TXA concentrations of 20, 50, 70 and 100 mg/ml. Cell cycle alterations and occurrence of cell death for apoptosis or necrosis were assessed by cytofluorimetry. Data were analyzed using Proc Mixed Procedure; LSMEANS was used to compare multiple group means with Tukey's honestly significant difference test. A significant correlation between the controlled for factors (type of treatment, time and concentration) was found in the performed experimen...Continue Reading

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Citations

Aug 30, 2020·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Scott M BolamJacob T Munro
Feb 26, 2021·The American Journal of Sports Medicine·William L JohnsNirav K Patel

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Methods Mentioned

BETA
ELISA
FACS
flow cytometry

Software Mentioned

SAS System
CellQuest
Modfit LT
LSMEANS
POWER

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis